FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520893
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTAGTCCTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCA20510.3937468923560117No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18820.3613026091730931No Hit
ATTTTATACAGCTCCACCAACAGAAACTACTTAGCTTGGTACCAGCAAAA18710.35919085109609844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18070.34690425864812924No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17310.3323139301161659No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG16000.307164811199229No Hit
TTATTACTGTCAGCAATATTATAGTAGTCCTCCGACATTCGGCCAAGGGA14930.2866231644502806No Hit
CTATAATATTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCT13880.2664654737153312No Hit
GTCTATTACTGTACAAGAGGGCAGGGTTCGGATGGCTGGTTCGGCCCCTG13580.2607061335053456No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13210.25360294724636345No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12480.23958855273539864No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12360.2372848166514044No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11790.22634207025243192No Hit
GTAATAGACAGCCGTGTCCTCGACTGTCAGACTGTCCATTTGCAGATACA11630.2232704221404396No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11470.22019877402844729No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC11460.2200067960214478No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10800.20733624755947958No Hit
CTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT9870.18948229290852442No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9580.18391493070553838No Hit
CAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCGGATGGTGAGAGT9260.1777716344815538No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9200.17661976643955668No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT9090.17450800836256197No Hit
CTTTATTACTGTCAGCAATATTATAGTAGTCCTCCGACATTCGGCCAAGG9040.1735481183275644No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9010.17297218430656583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8880.1704764702155721No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT8880.1704764702155721No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8560.1643331739915875No Hit
CTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA8550.164141195984588No Hit
TTATAGTAGTCCTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAAC7860.15089471350162126No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC7810.14993482346662368No Hit
GTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCGGATGGTGAGAGTGA7750.14878295542462655No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT7530.14455943927063714No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7150.13726427500465546No Hit
GTATAAAATATGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGAC7080.13592042895565884No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC6950.1334247148646651No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA6760.12977713273167427No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA6720.12900922070367618No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC6640.12747339664768004No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC6590.12651350661268243No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG6590.12651350661268243No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT6330.12152207843069499No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6220.11941032035370028No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC6090.11691460626270654No Hit
GTACAAGAGGGCAGGGTTCGGATGGCTGGTTCGGCCCCTGGGGCCAGGGA5880.11288306811571666No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG5860.11249911210171762No Hit
GTCTGACAGTCGAGGACACGGCTGTCTATTACTGTACAAGAGGGCAGGGT5740.1101953760177234No Hit
CTACTATAATATTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAG5720.10981142000372437No Hit
ACTATAATATTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGC5660.10865955196172727No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5660.10865955196172727No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTC5630.10808361794072871No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG5560.10673977189173207No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGACTTTGGGCTGAGCTGGG5540.10635581587773305No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC5430.10424405780073834No Hit
CCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGACGAA5430.10424405780073834No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC5420.10405207979373883No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC5330.10232427773074317No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCA5240.1005964756677475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3100.094.918451
GTATCAA9400.081.238461
GCATCGA500.001696888774.93345145
TCAACGC11050.069.1078344
TATCAAC11250.068.501992
ACGTGGG550.002476486668.12132145
CAACGCA11250.067.879245
ATCAACG11450.066.693583
AACGCAG11650.065.548636
ACGCAGA14000.054.0454037
CGCAGAG14250.053.0972378
TAGGACC954.107278E-851.622324
GCAGAGT14650.051.1692669
CTATTAG553.8101999E-450.9518971
GACCGTT702.075545E-550.0420387
CCGTTGA4000.043.786789
GTAGAAC658.6805696E-443.1131443
CCCGTTG4100.042.7188158
GTACATG19350.041.9991071
TACATGG19300.041.7449152
Produced by FastQC (version 0.11.5)