FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921743
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAGACGGCCGTGTCCTCGACTCTCAGGCTGCTCATTTCCAGATACA27070.293682729350806No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20480.22218774647596995No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19250.2088434628741417No Hit
GGCTAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA18520.20092368480151193No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18290.1984284122580806No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18210.19756049137340886No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17240.1870369506467638No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16190.17564548903544697No Hit
GTCTATTATTGTGCGAGAGTGCAGGTTTCCGCGGTGGCGACTACTGCGGG15810.17152286483325613No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14730.1598059328901874No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14620.15861254167376374No Hit
GTATGTGTCAGTCCCAGTAGAGCTAATGTATGAAACCCAGTCCAGCCCCT14610.15850405156317976No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13850.15025880315879805No Hit
CTCCAGGGACAACGCCAAGAATTCACTGTATCTGGAAATGAGCAGCCTGA13830.15004182293763013No Hit
GACACATACAACGCAGACTCTGTGGAGGGCCGATTCACCATCTCCAGGGA13440.1458107086248553No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGACT13300.14429184707667975No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13210.143315436081424No Hit
CTATTAGCCTGTTGACAATAGTAAGTTGCAGAATCCTCAGGCTGCAGGCT13170.14288147563908812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.139735262432153No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12690.1376739503310576No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCGTCACGCTTCAGCG12550.136155088782882No Hit
CAATAATAGACGGCCGTGTCCTCGACTCTCAGGCTGCTCATTTCCAGATA12320.1336598162394507No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12090.13116454369601938No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12000.13018813270076365No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11410.12378721617630946No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11240.12194288429638196No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT11160.1210749634117102No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11150.12096647330112624No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTGTTAGCAG11140.12085798319054225No Hit
GTACATGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT11030.1196645919741186No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11010.11944761175295066No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10450.11337216556024836No Hit
CTATTATTGTGCGAGAGTGCAGGTTTCCGCGGTGGCGACTACTGCGGGGA10390.11272122489674455No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10350.11228726445440865No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9990.10838162047338575No Hit
CTACTACATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGACTGGG9960.10805615014163385No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGGGACTACTACATGAGCTGGG9960.10805615014163385No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9810.1064287984828743No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9810.1064287984828743No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9640.1045844666029468No Hit
GTCCCTGGAGATGGTGAATCGGCCCTCCACAGAGTCTGCGTTGTATGTGT9620.10436748638177887No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9570.103825035828859No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9450.10252315450185137No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG9400.10198070394893154No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9330.10122127317484374No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG300.001989590271.999814
TCAACGC22850.050.1005254
ATCAACG23200.049.0396733
GAATACG4700.049.018499
TATCAAC25000.045.796852
GTGCTAG5600.043.7260321
CGCAGAG27050.042.585388
CTAGAGA6300.038.857044
GCAGAGT29700.038.785679
GCTAGAG6100.037.774493
CAGCGCT3650.037.4773189
GCAGCGC3900.035.0749248
TGCTAGA7750.034.377832
GTGTAGA4850.034.1535031
GTATCAA38350.033.2396971
GTACATG26200.031.0617771
TACATGG25600.030.9407752
CAACGCA38200.030.9092375
AACGCAG38750.030.4705246
ACATGGG26650.030.2621153
Produced by FastQC (version 0.11.5)