FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921743
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAGACGGCCGTGTCCTCGACTCTCAGGCTGCTCATTTCCAGATACA26320.2855459710570083No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23060.2501781950066342No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19830.21513588928801197No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17860.19376333750296992No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17630.1912680649595386No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17560.19050863418545083No Hit
GGCTAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA17130.18584355943034012No Hit
GTCTATTATTGTGCGAGAGTGCAGGTTTCCGCGGTGGCGACTACTGCGGG16170.17542850881427904No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16020.1738011571555195No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15490.1680511812945691No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15470.16783420107340116No Hit
CTCCAGGGACAACGCCAAGAATTCACTGTATCTGGAAATGAGCAGCCTGA14580.15817858123142783No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13980.15166917459638968No Hit
GTATGTGTCAGTCCCAGTAGAGCTAATGTATGAAACCCAGTCCAGCCCCT13970.1515606844858057No Hit
CTATTAGCCTGTTGACAATAGTAAGTTGCAGAATCCTCAGGCTGCAGGCT13770.1493908822741263No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13550.14700409984127896No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13330.14461731740843164No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCGTCACGCTTCAGCG13240.1436409064131759No Hit
GACACATACAACGCAGACTCTGTGGAGGGCCGATTCACCATCTCCAGGGA13180.14298996574967207No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGACT12770.13854187121572933No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12450.1350701876770423No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12120.1314900140277713No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12050.13073058325368353No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT11670.12660795905149266No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11380.12346174584455755No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11200.12150892385404607No Hit
CAATAATAGACGGCCGTGTCCTCGACTCTCAGGCTGCTCATTTCCAGATA11190.12140043374346211No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11080.12020704252703844No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11080.12020704252703844No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11060.11999006230587052No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGGGACTACTACATGAGCTGGG10310.11185330401207279No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10270.11141934356973691No Hit
CTACTACATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGACTGGG10100.10957501168980942No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT10070.10924954135805752No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTGTTAGCAG10050.10903256113688957No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10040.10892407102630561No Hit
CTATTATTGTGCGAGAGTGCAGGTTTCCGCGGTGGCGACTACTGCGGGGA10030.10881558091572162No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10000.1084901105839697No Hit
GTACATGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT9960.10805615014163385No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9890.10729671936754603No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9810.1064287984828743No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9730.10556087759820254No Hit
GTCCCTGGAGATGGTGAATCGGCCCTCCACAGAGTCTGCGTTGTATGTGT9710.10534389737703459No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9620.10436748638177887No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9290.10078731273250788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG3700.050.5887229
GTACGGA605.0989416E-448.033531
GTGCTAG4650.043.3851281
GCAGCGC4900.042.6072968
CAGCGCT4700.041.3569039
GGCAGCG4750.040.9215667
TAGGACG750.00153223638.412224
CTAGAGA5800.036.0114564
GTACACG9050.035.8105555
TACACGG9000.035.1959156
GCTAGAG5600.033.442843
ACACGGG9850.032.1587037
ACGGGGT10150.031.9174779
ATCAACG18200.030.8703143
CATGGGG11300.030.5938034
TCAACGC18400.030.5314524
GTATGTA11750.030.0465091
TCTACAC9700.029.7033963
TGCTAGA7200.029.0139542
TATCAAC20250.028.813862
Produced by FastQC (version 0.11.5)