FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918225
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGGGGATGTACACTTTTGGCCAGGGGACCAAGC36720.39990198480764516No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT25150.27389800974706635No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT21040.22913773857170086No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20410.2222766751068638No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC19510.21247515587138227No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17720.19298102316970242No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15960.17381360777587193No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14910.1623785020011435No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14550.1584578943069509No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC14340.15617087315200523No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT14140.1539927577663427No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12620.13743908083530726No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12170.13253832121756648No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA12160.13242941544828338No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG12010.13079582890903646No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11770.1281820904462414No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCTCCGGGGATGTACACTTTTG11510.12535054044488006No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11440.12458820005989818No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11390.12404367121348255No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTG10920.11892510005717552No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT10900.11870728851860927No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10380.11304418851588664No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10210.11119279043807345No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10140.11043045005309157No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10110.11010373274524217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG14100.056.7001383
TCAACGC14450.055.326784
GTATCAA15200.054.5041051
TATCAAC15950.050.5751952
CAACGCA16000.049.964275
AACGCAG17300.046.6133356
ACGCAGA17400.045.931647
CGCAGAG17700.045.153148
GGTATCA7600.038.8637961
GCAGAGT22600.035.36339
GTCATAG10650.033.8216931
GTACATG25950.029.704441
CAGAGCG8900.029.123989
ACATGGG25100.028.981973
AATCCGC1504.6104324E-528.8003836
TACATGG25650.028.3605252
ATAGGTG10500.028.1238544
ACACAGT11100.027.8922626
CCTATAC2654.8876245E-927.1790543
GAATACG2654.9058144E-927.170179
Produced by FastQC (version 0.11.5)