FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671216
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA19650.29275225858739956No Hit
GTATATTACTGTGCGGCCAACCGCCTGCCATGGGCTTTTGATCTCTGGGG15300.22794450668637217No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14710.21915448976186505No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9990.14883435436580775No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9570.14257705418226027No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9380.13974637076589355No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8990.13393602059545662No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8620.12842363710042667No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACAGCG8470.12618888703487402No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8360.1245500703201354No Hit
ATATTACTGTGCGGCCAACCGCCTGCCATGGGCTTTTGATCTCTGGGGCC7830.11665395342184931No Hit
GCCGTATATTACTGTGCGGCCAACCGCCTGCCATGGGCTTTTGATCTCTG7690.11456818669400015No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7620.11352530333007557No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7430.11069461991370885No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7330.10920478653667373No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7280.10845986984815618No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7230.10771495315963861No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC350.00366269161.724541
TCAACGC6650.059.5543254
ATCAACG6900.057.3965643
GTATCAA7650.053.6559751
TATCAAC7650.052.710712
CAACGCA7900.050.1311765
AACGCAG8350.047.425966
GGTATCA2800.046.2934041
GTACATG12700.035.722471
TACATGG12950.035.0302352
GCAGAGT11700.034.459569
ATACGGC9950.032.563376
GTCTATA1551.5433561E-632.5215341
TATACGG10000.032.402975
CGCAGAG12300.032.193278
TTTCGCG950.00489027530.3140499
GGCAGCG3850.029.9225887
TACGGCC11000.029.4550487
GGTATAC1504.6061436E-528.8026393
AATATAC12350.027.9863683
Produced by FastQC (version 0.11.5)