FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355298
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT31440.23197850214491572No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30990.22865819915620034No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC30240.22312436084167467No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28090.2072606910067011No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27530.20312875839852196No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26920.1986279032360411No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24780.18283801791192786No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23520.17354116954352475No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23140.17073735813083174No Hit
CTGTAAAAGTAGCCGCTCGCACAGAAATACACGGCCGTGTCCTCAGATCT23050.17007329753308867No Hit
GTATCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT22850.16859760731588183No Hit
GTATTACTGTCAACAGTATGCTACCTCACCTACTTTTGGCCCGGGGACCC22670.16726948612039566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22490.1659413649249095No Hit
CTTTTACAGTGGGTCCGATCTGTCCTTCCACGGCATGGACGTCTGGGGCC22080.16291619997963547No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21860.16129294074070796No Hit
GTCTGGGGCTGAGCTGAAGAAGCCTGGGTCCTCGGTGAGGGTCTCCTGCA21670.15989103503436145No Hit
GTACCATAGTCACCGTCTCCGCAGCCTCCACCAAGGGCCCATCGGTCTTC21320.15730857715424948No Hit
CTATGGTACCTTGGCCCCAGACGTCCATGCCGTGGAAGGACAGATCGGAC19360.1428468130256224No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC18240.13458294780926408No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17830.13155778286399006No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG17640.13015587715764354No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17260.12735206574495056No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG16990.12535988395172132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.12462203884311791No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16500.12174444291956456No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16270.12004739916977668No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16250.11989983014805601No Hit
GAAATACACGGCCGTGTCCTCAGATCTCAGGCTGGTCAGCTCCATGTGGG16200.11953090759375427No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA16110.1188668469960112No Hit
GTGTATTACTGTCAACAGTATGCTACCTCACCTACTTTTGGCCCGGGGAC15940.1176125103113854No Hit
CACGAATACAGCCCACATGGAGCTGACCAGCCTGAGATCTGAGGACACGG15860.11702223422450267No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15780.11643195813761992No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15630.11532519047471479No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15050.11104568884481494No Hit
GTATTCGTGAATGCGTCCGCGGTAATGCTGACTCTGGCCTGGAACTTCTG14970.1104554127579322No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG14860.10964378313846844No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14400.10624969563889271No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG14270.10529049699770825No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC14050.10366723775878074No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13710.1011585643895291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27650.066.010091
ATCAACG31650.057.5887763
TCAACGC31650.056.9059074
GGTATCA12050.056.2763331
CAACGCA32200.056.151435
TATCAAC33050.055.149312
AACGCAG33700.054.2412876
CGCAGAG37750.048.2313848
ACGCAGA42950.042.391967
TATAACG700.001090173441.167363
ATACCGT1553.7056452E-837.143646
CAGCGCT7650.036.688459
GTACGAC800.002101092436.0214423
TACCGTC1301.7411357E-533.2149857
GCAGAGT57650.031.9570589
AGCGCTG7950.031.7134785
GTACACG14600.031.5769425
GCAGCGC9400.031.3893368
GATACCG1952.759698E-729.5527785
TACATGG35100.029.3508032
Produced by FastQC (version 0.11.5)