FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507559
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCAGATCTCAGGGTCCTCAGTTCCAGGTAGG13320.26243254478789657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.20312909435159263No Hit
GTCTATTACTGTGCGATGAGTTCTGATGTGACGATTTTTCACGTGGGCCG8730.17199970840828357No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT6190.12195626518296396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.10658859364133036No Hit
GTCTGGAACTGTGGTGGAGAGGCCTGGGGCCTCAGTGAGGGTCTCCTGCA5280.1040273150510581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.10008688645063923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5200.063.7407151
GTATCAA11400.062.573831
ATCAACG12250.056.4395453
TCAACGC12350.055.9660034
CAACGCA12750.054.2102055
AACGCAG13500.051.198536
TATCAAC13700.050.466022
CGCAGAG14650.047.179538
GAATACG1151.4899342E-743.8248339
ACGCAGA16950.041.202367
TAGACGG3650.037.4783745
AGACGGC3950.034.6319166
GCAGAGT20600.033.5524339
ATAGACG4150.032.9629064
GTGCTAG2452.1009328E-929.4100911
GTATAGA2853.45608E-1027.810591
CAATACG1050.00797066827.4277844
GTAATAG5800.027.33111
GTACATG14250.026.7992971
AATAGAC5450.026.429073
Produced by FastQC (version 0.11.5)