FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976763
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24320.2489856802520161No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA24240.2481666484090818No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC23250.23803112935277035No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA19930.2040413078709984No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19110.1956462314809222No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGAAG18900.19349627289321975No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17650.18069890034737188No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17100.17506805642719883No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15800.16175878897951704No Hit
AGCCAGGACAGGCCCCTCTGCTTGTCATCTACGGTAAAAACACCCGGCCC15550.15919931447034746No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15500.15868741956851354No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14930.15285181768760694No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14820.15172564890357232No Hit
ACCTGGTACCAGCAGAAGCCAGGACAGGCCCCTCTGCTTGTCATCTACGG13890.1422044037294615No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13750.14077109800432655No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGA13690.14015682412212585No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT13560.13882589737735765No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC13500.13821162349515695No Hit
GACTATTACTGTCAGTCCCGGGACAGCAGTGGTAGCTGGGTGTTCGGCGG13430.13749497063258947No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13310.1362664228681881No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13070.1338093273393853No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12990.13299029549645103No Hit
CTATTACTGTCAGTCCCGGGACAGCAGTGGTAGCTGGGTGTTCGGCGGAG12940.1324784005946171No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12690.12991892608544756No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12690.12991892608544756No Hit
GTGTAAGACTGCCTCGCACAGTAATACACGGCCGTGTCCGCGGCGGTCAC12660.1296117891443472No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCTACCACTGC12550.12848562036031258No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCC12450.12746183055664476No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG12340.12633566177261013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12220.12510711400820876No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12090.12377618726344057No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG11940.12224050255793882No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11890.12172860765610491No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11860.12142147071500457No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11840.121216712754271No Hit
GTCTCTGGTGACTCCATCACCAATAGTCAGTGGTGGAGTTGGGTCCGCCA11760.12039768091133674No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11660.11937389110766891No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11580.11855485926473464No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11520.11794058538253394No Hit
GTCCTGGCTTCTGCTGGTACCAGGTTGCATAATAATTTCTGAGGCTGTCT11450.11722393251996646No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11350.11620014271629864No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT11310.1157906267948315No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT11250.1151763529126308No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA11120.11384542616786261No Hit
CACTGGGACTCAGGCGGAAGATGAGGCTGACTATTACTGTCAGTCCCGGG10660.10913599307099062No Hit
TTATTATGCAACCTGGTACCAGCAGAAGCCAGGACAGGCCCCTCTGCTTG10540.1079074453065892No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCTACCACTG10530.10780506632622243No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10400.10647413958145426No Hit
GCCCTCAGGGATCCCAGACCGACTCTCTGGCTCCAGTTCAGGAAACACAG10010.10248135934714973No Hit
GTCAGGATCACATGCCAAGGAGACAGCCTCAGAAATTATTATGCAACCTG9810.10043377973981407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG2750.044.5051359
TATACCG800.00210787335.9968035
TATAACG800.00210787335.9968033
ACCGACC2551.8189894E-1233.879348
GTGCTAG4050.033.864921
GTATCAA44850.032.3508031
ATAACGC1153.2947233E-431.3015654
ATCAACG46550.031.086393
TCAACGC47000.030.941934
CAACGCA47200.030.8108225
CACCGAC3050.030.6857997
AACGCAG47700.030.4878566
TATCAAC47600.030.1228352
CGCAGAG50600.028.598258
ACGCAGA51150.028.431497
GCACCGA3403.6379788E-1227.5269646
TACACCG1050.00797655827.4261345
GGTTTAT11300.025.5524791
GCTAGAG5100.025.4095063
TATTGAG14850.025.209885
Produced by FastQC (version 0.11.5)