FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976763
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21700.22216238739591895No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA20310.20793170912493616No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC18330.18766067101231312No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA16920.17322523478059673No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGAAG16550.16943721250702576No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15950.1632944736850188No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14520.14865427949256882No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14100.14435436231716392No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13710.1403615820828594No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13140.13452598020195278No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13000.13309267447681783No Hit
AGCCAGGACAGGCCCCTCTGCTTGTCATCTACGGTAAAAACACCCGGCCC12560.12858799934067935No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12260.12551662992967588No Hit
GACTATTACTGTCAGTCCCGGGACAGCAGTGGTAGCTGGGTGTTCGGCGG11640.11916913314693534No Hit
ACCTGGTACCAGCAGAAGCCAGGACAGGCCCCTCTGCTTGTCATCTACGG11590.11865723824510144No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC11520.11794058538253394No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT11490.11763344844143359No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGA11330.11599538475556506No Hit
GTGTAAGACTGCCTCGCACAGTAATACACGGCCGTGTCCGCGGCGGTCAC11250.1151763529126308No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11180.11445970005006331No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11150.11415256310896298No Hit
CTATTACTGTCAGTCCCGGGACAGCAGTGGTAGCTGGGTGTTCGGCGGAG11120.11384542616786261No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11070.1133335312660287No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10970.11230974146236089No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10910.11169546758016018No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10590.10841934020842314No Hit
GTCCTGGCTTCTGCTGGTACCAGGTTGCATAATAATTTCTGAGGCTGTCT10490.10739555040475529No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10410.10657651856182104No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10260.1050408338563193No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA10100.10340277017045077No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10070.1030956332293504No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10020.1025837383275165No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9960.1019694644453158No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG9930.10166232750421546No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9880.10115043260238155No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG9820.10053615872018085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC3750.036.4734048
TGCACCG4300.033.482315
ATAGACG950.00489431130.3103058
CCGACCG4400.029.4492179
CGTAATA1000.006288811628.794792
CACCGAC4800.028.4948437
CTATACC2054.2977808E-728.0924764
TATTGAG13000.027.133555
GCACCGA4250.027.1009776
ATACACG12650.025.0389484
GTACATG44250.024.4236181
GTATAGG2401.6870563E-624.0165561
TCTACAC8500.023.7133563
TACATGG44400.023.671392
GTAATAC13500.023.4828571
ATGGGAG13600.023.2899045
GTATAGA3102.582965E-823.241831
TACACGG13400.023.1002985
TTATTGA15050.022.9593014
TCGCTGC5050.022.827621
Produced by FastQC (version 0.11.5)