FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729872
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTTAGACAGTGGGGGCCCAACAAGGTTCAGCGGCAGTGGATCTGGGAC58730.8046616393011378No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34100.4672052085845189No Hit
GTCTAAAGTAGATGCCTTGTAGATCAGGAGTTTAGGGGCTCTCCCTGGCT32880.4504899489225508No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23250.3185490058530811No Hit
GTCTAAATATTTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAAATCA21450.2938871473354232No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20880.28607755880483154No Hit
TATTTAGACTGTTGGCAGTAATAAATTCCAACATCCTCGGACTGCAGGCT19320.264703948089528No Hit
GTAATAAACGGCCGTGTCCTCGGCGCTCAGGCTGTTCATTTGCAGATAGA16810.2303143564899051No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16790.23004033583970887No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15010.20565249797224722No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG15000.20551548764714908No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14910.20428239472126622No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14450.19797991976675364No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14120.19345857903851635No Hit
GTGGGGGCCCAACAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACT13680.18743012473419995No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGAGTGTTAGTCC13590.1861970318083171No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA13240.1814016704298836No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT13140.1800315671789026No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12930.17715435035184252No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12230.16756362759497556No Hit
GGTATCAGCAGAAGCCAGGGAGAGCCCCTAAACTCCTGATCTACAAGGCA12170.16674156564438694No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12020.16468641076791546No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT11370.15578073963653902No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGA11370.15578073963653902No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATGTTGGAATTTA11300.15482166736085232No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11280.15454764671065613No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11190.1533145537847732No Hit
GTTTATTACTGTGTGAAGGATACCTATCATGATGGTAGTCACTTCTTAGA11000.15071135760790932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10550.14454589297849485No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTCCGCCGAAAGTG10450.14317578972751385No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10370.14207970712672907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10310.14125764517614048No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10180.13947651094986518No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATG10110.13851743867417848No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9990.1368733147730013No Hit
GTGTGAAGGATACCTATCATGATGGTAGTCACTTCTTAGACTACTGGGGC9860.135092180546726No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9720.1331740359953526No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9670.13248898436986212No Hit
CACCATCAGCAGCCTGCAGTCCGAGGATGTTGGAATTTATTACTGCCAAC9600.13152991209417542No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9480.12988578819299823No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT9450.12947475721770393No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9200.12604949909025143No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG9120.12495341648946666No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC9100.12467939583927046No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9090.12454238551417235No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9010.12344630291338755Clontech Universal Primer Mix Long (96% over 26bp)
GAACAGAGAGAGCTCACCATGGAGTTTGGGCTGCGCTGGCTTTTTCTTGT8980.12303527193809326No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT8510.11659578665848258No Hit
TACTAATAGTGGTGGAAAGACATTCTACGCAGACTCCGTGAAGGGCCGGT8050.11029331170397001No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG7960.10906021877808711No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG7950.10892320845298901No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7910.10837516715259661No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7900.10823815682749852No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7900.10823815682749852No Hit
GCCAGGGAGAGCCCCTAAACTCCTGATCTACAAGGCATCTACTTTAGACA7710.10563496065063463No Hit
TCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCC7630.10453887804984985No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7350.10070258894710306No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCGCCTTTT7340.10056557862200496No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)