FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729872
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTTAGACAGTGGGGGCCCAACAAGGTTCAGCGGCAGTGGATCTGGGAC46360.6351798671547888No Hit
GTCTAAAGTAGATGCCTTGTAGATCAGGAGTTTAGGGGCTCTCCCTGGCT29420.4030843764386084No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28110.3851360238507574No Hit
GTCTAAATATTTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAAATCA20920.2866256001052239No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18280.25045487427932567No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17520.24004208957187015No Hit
TATTTAGACTGTTGGCAGTAATAAATTCCAACATCCTCGGACTGCAGGCT16040.21976456145735143No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13740.18825218668478857No Hit
GTAATAAACGGCCGTGTCCTCGGCGCTCAGGCTGTTCATTTGCAGATAGA13480.184689918232238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13250.1815386807549817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12120.16605651401889648No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT11840.16222022491614968No Hit
GTGGGGGCCCAACAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACT11530.1579729048381086No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11230.15386259508516562No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA11060.15153341955849794No Hit
GTTTATTACTGTGTGAAGGATACCTATCATGATGGTAGTCACTTCTTAGA10840.14851919240633976No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10760.14742310980555495No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGAGTGTTAGTCC10710.14673805818006444No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10550.14454589297849485No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT10550.14454589297849485No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10450.14317578972751385No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT10380.14221671745182718No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATGTTGGAATTTA10330.14153166582633667No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGA9700.1329000153451564No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9680.1326259946949602No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9400.12878970559221342No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9280.12714558169103624No Hit
GGTATCAGCAGAAGCCAGGGAGAGCCCCTAAACTCCTGATCTACAAGGCA9180.12577547844005524No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTCCGCCGAAAGTG9130.12509042681456475No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9120.12495341648946666No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA8810.12070609641142557No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA8770.12015805511103317No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC8640.11837692088475786No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATG8370.1146776421071092No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGTTGGGCCCCCACTGTCTAAAGT8160.11180042528004909No Hit
GTGTGAAGGATACCTATCATGATGGTAGTCACTTCTTAGACTACTGGGGC8070.11056733235416621No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8010.10974527040357761No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC7820.10714207422671373No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7760.10632001227612513No Hit
GAACAGAGAGAGCTCACCATGGAGTTTGGGCTGCGCTGGCTTTTTCTTGT7620.10440186772475174No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7590.10399083674945744No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7520.10303176447377074No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7450.10207269219808404No Hit
CACCATCAGCAGCCTGCAGTCCGAGGATGTTGGAATTTATTACTGCCAAC7450.10207269219808404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG2600.049.8880771
GAATACG2500.048.950829
CAATACG450.00991255847.9914
TAGACCG657.5966143E-444.299395
AGTCCCG700.001094047841.1351478
AAGCGCT2151.2732926E-1136.8303034
TAGATGC17450.035.477599
CTAAAGT17550.034.8652573
TAAAGTA18500.034.6313484
GCCTAAG1051.9206257E-434.3145521
GTACACG5700.034.098875
AGTAGAT18400.034.0370987
TCTAAAG18100.033.40812
AAGTAGA19500.032.8553776
CTAGAGA3550.032.4446224
GTAGATG19100.032.412778
TACACGG6000.032.3939256
ACGGGGT5800.032.2698149
AGAATAC3850.031.786258
GATTCAC2757.2759576E-1231.4446031
Produced by FastQC (version 0.11.5)