FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929370
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA65110.7005821147659167No Hit
GTTTATTACTGTGCGAGACAAGGTGCAGGGGCGGATCTCAGCTCCGGGCT45510.48968656186448883No Hit
GTCTCTGGTGGCTCCATCAGCCGTAGTAGTTACTACTGGGGCTGGGTCCG45060.4848445721295071No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36280.39037197241141847No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC31210.3358188880639573No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA30640.32968570106631373No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27660.2976209690435456No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27470.29557657337766446No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25730.27685421306906827No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23880.25694825526969883No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23610.25404306142870975No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT23210.2497390705531704No Hit
GTAGTAGGTGTTCCCACTATGATTGATGCTGCCAATCCACTCCAGGCCCT21360.22983311275380094No Hit
TCTTAGACGTGTCTACCGATACGGTGACTCGACTGATGAGGGACGCGTTG19180.20637636248211155No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18890.20325596909734553No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17880.19238839213660866No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG17750.1909895951020584No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17580.18916039897995415No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT17480.18808440126106934No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCCGTAGTAGTTACTACTGGG17330.18647040468274206No Hit
ACCTACTACAACGCGTCCCTCATCAGTCGAGTCACCGTATCGGTAGACAC17310.1862552051389651No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA17260.1857172062795227No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17200.18507160764819178No Hit
TTATTACTGTGCGAGACAAGGTGCAGGGGCGGATCTCAGCTCCGGGCTTG16960.18248921312286817No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16920.18205881403531424No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC16120.17345083228423555No Hit
CGTCTAAGAAGCAGATCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGAC16070.17291283342479313No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16070.17291283342479313No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG15900.1710836373026889No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT15730.1692544411805847No Hit
GTGCAGGGGCGGATCTCAGCTCCGGGCTTGGGGCATTCGACCCCTGGGGC15020.16161485737650236No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14940.1607540592013945No Hit
AGTTACTACTGGGGCTGGGTCCGCCAGCCCCCAGGGAAGGGCCTGGAGTG14790.15914006262306724No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC14700.15817166467607088No Hit
CAGTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA14440.1553740706069703No Hit
CCCCTGCACCTTGTCTCGCACAGTAATAAACAGCCGTGTCTGCGGCGGTC14190.15268407630975822No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG14170.15246887676598125No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC14010.15074728041576552No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG13770.1481648858904419No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC13750.14794968634666494No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA13390.14407609455867953No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTC13100.1409557011739135No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA13100.1409557011739135No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT12740.1370821093859281No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12610.13568331235137782No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12500.1344997148606045No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC12230.13159452101961544No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT12130.1305185233007306No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG11900.12804372854729548No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC11840.12739812991596458No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11760.1265373317408567No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11710.1259993328814143No Hit
CTACTGGGGCTGGGTCCGCCAGCCCCCAGGGAAGGGCCTGGAGTGGATTG11700.1258917331095258No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11620.12503093493441794No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG11570.12449293607497552No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11520.1239549372155331No Hit
GTCCCAGCTACAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGG11320.12180294177776343No Hit
CCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTTTATTACTGT11250.12104974337454405No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA11140.11986614588377073No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCCGTAGTAGTTACT11050.11889774793677438No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA11050.11889774793677438No Hit
GGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTT10980.11814454953355498No Hit
ATCATAGTGGGAACACCTACTACAACGCGTCCCTCATCAGTCGAGTCACC10900.11728375135844711No Hit
CCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGT10770.11588495432389684No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA10620.11427095774556958No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC10600.11405575820179262No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10560.11362535911423868No Hit
TCCCTCATCAGTCGAGTCACCGTATCGGTAGACACGTCTAAGAAGCAGAT10410.11201136253591142No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC10390.11179616299213445No Hit
GTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGAC10350.11136576390458053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.10856816983547996No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10040.10803017097603754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10020.10781497143226057No Hit
GTATCAACGCAGAGTACATGGGGCTTTCTGAGAGTCATGGATCTCATGTG10000.1075997718884836No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGC9960.10716937280092965No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9960.10716937280092965No Hit
CACCTACTACAACGCGTCCCTCATCAGTCGAGTCACCGTATCGGTAGACA9960.10716937280092965No Hit
CCTACTACAACGCGTCCCTCATCAGTCGAGTCACCGTATCGGTAGACACG9910.10663137394148724No Hit
GGCCTGGAGTGGATTGGCAGCATCAATCATAGTGGGAACACCTACTACAA9740.10480217781938303No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG9700.10437177873182908No Hit
CATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTC9660.10394137964427515No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC9370.10082098625950912No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGG9310.10017538762817824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)