FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929370
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA64500.6940185286807192No Hit
GTTTATTACTGTGCGAGACAAGGTGCAGGGGCGGATCTCAGCTCCGGGCT45360.4880725652861616No Hit
GTCTCTGGTGGCTCCATCAGCCGTAGTAGTTACTACTGGGGCTGGGTCCG42950.46214102026103704No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT38870.41824031333053574No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA31420.33807848327361545No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC29530.3177421263866921No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28850.31042534189827514No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26860.28901298729246694No Hit
GTAGTAGGTGTTCCCACTATGATTGATGCTGCCAATCCACTCCAGGCCCT24600.26469543884566965No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23810.2561950568664794No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23620.25415066120059826No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23550.2533974627973789No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21690.2333839052261209No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20790.2236999257561574No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20200.21735153921473688No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT19830.21337034765486299No Hit
TCTTAGACGTGTCTACCGATACGGTGACTCGACTGATGAGGGACGCGTTG19370.20842075814799274No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17920.1928187912241626No Hit
ACCTACTACAACGCGTCCCTCATCAGTCGAGTCACCGTATCGGTAGACAC17670.1901287969269505No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17660.19002119715506202No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17450.18776160194540387No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17230.18539440696385723No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT17220.18528680719196874No Hit
TTATTACTGTGCGAGACAAGGTGCAGGGGCGGATCTCAGCTCCGGGCTTG16420.17667882544089006No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCCGTAGTAGTTACTACTGGG16340.1758180272657822No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15850.1705456384432465No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC15710.16903924163680772No Hit
CGTCTAAGAAGCAGATCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGAC15670.1686088425492538No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG15640.16828604323358834No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15420.1659188482520417No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15040.16183005692027932No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15020.16161485737650236No Hit
GTGCAGGGGCGGATCTCAGCTCCGGGCTTGGGGCATTCGACCCCTGGGGC14670.15784886536040543No Hit
CCCCTGCACCTTGTCTCGCACAGTAATAAACAGCCGTGTCTGCGGCGGTC14620.15731086650096301No Hit
AGTTACTACTGGGGCTGGGTCCGCCAGCCCCCAGGGAAGGGCCTGGAGTG14460.15558927015074728No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT14340.15429807288808547No Hit
CAGTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA14000.15063968064387703No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13910.14967128269688068No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13850.14902568406554978No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC13510.14536729182134134No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC13170.1417088995771329No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG13130.14127850048957896No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTC13120.14117090071769048No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA12840.13815810710481294No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12590.13546811280760085No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT12490.134392115088716No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12480.13428451531682753No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12220.13148692124772696No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC12100.13019572398506515No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG11780.12675253128463368No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC11780.12675253128463368No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG11570.12449293607497552No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC11490.12363213789986766No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA11440.12309413904042522No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11410.12277133972475977No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC11240.12094214360265557No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA11170.12018894519943617No Hit
CCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTTTATTACTGT11130.11975854611188225No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA11040.1187901481648859No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11000.11835974907733196No Hit
GTCCCAGCTACAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGG10980.11814454953355498No Hit
TCCCTCATCAGTCGAGTCACCGTATCGGTAGACACGTCTAAGAAGCAGAT10660.1147013568331235No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10590.11394815842990413No Hit
CTACTGGGGCTGGGTCCGCCAGCCCCCAGGGAAGGGCCTGGAGTGGATTG10570.11373295888612717No Hit
ATCATAGTGGGAACACCTACTACAACGCGTCCCTCATCAGTCGAGTCACC10540.11341015957046172No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10370.1115809634483575No Hit
GGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTT10350.11136576390458053No Hit
CACCTACTACAACGCGTCCCTCATCAGTCGAGTCACCGTATCGGTAGACA10340.11125816413269204No Hit
GTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCCGTAGTAGTTACT10310.1109353648170266No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCAC10280.11061256550136114No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGC10170.10942896801058781No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC9970.10727697257281814No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCT9890.10641617439771027No Hit
CCTACTACAACGCGTCCCTCATCAGTCGAGTCACCGTATCGGTAGACACG9830.10577057576637938No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9760.10501737736316No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT9740.10480217781938303No Hit
GTATCAACGCAGAGTACATGGGGCTTTCTGAGAGTCATGGATCTCATGTG9740.10480217781938303No Hit
GGCCTGGAGTGGATTGGCAGCATCAATCATAGTGGGAACACCTACTACAA9630.1036185803286097No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGG9590.10318818124105578No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9450.10168178443461699No Hit
GTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTT9400.10114378557517457No Hit
GTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGAC9370.10082098625950912No Hit
GCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCTGCCACCTGCT9350.10060578671573217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)