FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744538
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGTCTTCAGGCTGTTCATTTGCAGATACG20030.2690258925669341No Hit
AATATATGCTGCGTCGCTGAAAGGCAGGTTCACCATCTCCAGAGATGATT13910.18682726737923383No Hit
GTGTATTACTGTGCAGCAGTAGTTGGTCCTCCAGGGGACTACTTTGACTA13360.179440136030666No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA12590.16909815214267102No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT12530.1682922832682818No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12520.16815797178921693No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11690.15701011902683276No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT10990.14760831549229186No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10870.14599657774351343No Hit
GCTATGCACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGGTTGG10620.14263879076689168No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT10400.13968393822746455No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9450.12692434771630193No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9290.12477536405126399No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT9040.12141757707464226No Hit
CTCCAGAGATGATTCAAGAAACACGGCGTATCTGCAAATGAACAGCCTGA8920.11980583932586382No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA8530.11456769164233391No Hit
TCTCTGGAGATGGTGAACCTGCCTTTCAGCGACGCAGCATATATTGGCGC8530.11456769164233391No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8410.11295595389355546No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG8290.11134421614477703No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG8030.10785211768909041No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8010.10758349473096067No Hit
GTATTACTGTGCAGCAGTAGTTGGTCCTCCAGGGGACTACTTTGACTACT7950.10677762585657145No Hit
GCATATATTGGCGCGTTATTGTCAGATGTGCTTCTGATACGGCCAACCCA7860.10556882254498763No Hit
GTCTTCAGGCTGTTCATTTGCAGATACGCCGTGTTTCTTGAATCATCTCT7640.1026139700055605No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7530.10113654373584693No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG2600.035.993429
ACGGGGT8150.034.447699
ACACGGG7950.034.4088027
TATGTAC7700.032.7212872
GTACACG7950.032.5978135
GTGCTAG3500.030.8867651
TCAACGC15950.030.6903114
ATCAACG16700.028.8809473
CAACGCA16500.028.7947355
TATCAAC16900.028.539162
CACGGGG9750.028.0564068
TCTACAC9000.027.9948793
ACCGCTC1306.7562086E-427.6872448
GTATGTA9150.027.5674211
GTGTTAC1607.108482E-527.025921
AACGCAG17600.026.9950646
GTATCAA19050.026.4820881
TTATTGA7500.025.9152624
CTAGAGA4550.025.3140534
TCCTATA2301.1728844E-625.03892
Produced by FastQC (version 0.11.5)