FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115619
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTATATATCACCAACACAGGGGCCTGGTCTGGCTTCTGCTGGTACC52330.4690669484833084No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT35260.31605772221520073No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA32150.28818082158873237No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC28950.2594971939344884No Hit
TATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTC23800.21333448067843952No Hit
ATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACAGGG23210.20804593682968828No Hit
TGATTATACCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGCCTGGAGT22710.20356412000871266No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATGAGTACCA21840.19576575874021507No Hit
GTATAATCATCAAAGGTCAATCCAGAGGCTACACAGGAGAGTCTCAGGGA21630.19388339567540533No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20770.18617467074332725No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAGCGTGA20750.18599539807048823No Hit
GTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG19870.17810740046557114No Hit
TTGTATTACTGTGCAAAAGAAGAGGGCGTCTGGGGCCAAGGGACCACGGT18610.16681322207671256No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG18400.1649308590119028No Hit
ATAATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA18240.1634966776291906No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17670.1583874064532784No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17530.15713249774340524No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17100.1532781352773662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.1497823181570052No Hit
GACTATTACTGTCAATCATCAGACAGAAGTGGTACTCATTGGGTGTTCGG16300.14610722836380519No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA16220.1453901376724491No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC16040.14377668361689788No Hit
CACCAACACAGGGGCCTGGTCTGGCTTCTGCTGGTACCAATAAGCAAATT16000.14341813827121985No Hit
GCCTTGTATTACTGTGCAAAAGAAGAGGGCGTCTGGGGCCAAGGGACCAC15800.1416254115428296No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15730.140997957187893No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15700.14072904817863446No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15640.1401912301601174No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15540.13929486679592226No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG15210.13633686769407835No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14900.13355814126507348No Hit
CCCAATGAGTACCACTTCTGTCTGATGATTGACAGTAATAGTCAGCCTCG14570.13060014216322957No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14350.12862814276200027No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA14230.12755250672496612No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA14090.12629759801509297No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13750.12324996257682955No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13730.12307068990399053No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGGCAAAG13420.12029196347498564No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13180.11814069140091733No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13120.11760287338240026No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG12970.11625832833610757No Hit
GTCTGAGAGCTGAGGACACGGCCTTGTATTACTGTGCAAAAGAAGAGGGC12810.11482414695339538No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12720.11401741992561978No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12620.11312105656142465No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12600.11294178388858561No Hit
CAGTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATA12550.11249360220648805No Hit
TATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGC12430.1114179661694539No Hit
GTATTACTGTGCAAAAGAAGAGGGCGTCTGGGGCCAAGGGACCACGGTCA12250.1098045121139027No Hit
CTTTATATATCACCAACACAGGGGCCTGGTCTGGCTTCTGCTGGTACCAA12150.10890814874970757No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12120.10863923974044902No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG12070.10819105805835146No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12000.10756360370341488No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11980.10738433103057585No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11960.10720505835773682No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11880.10648796766638073No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11870.10639833132996121No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11720.10505378628366854No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11580.10379887757379536No Hit
GCCTATTATACCACTTTTCCAACTAATACCTGAGACCCACTCCAGGCCCT11490.10299215054601973No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTAT11450.10263360520034169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29000.061.3496551
ATCAACG31650.053.912953
TCAACGC31700.053.827914
TATCAAC32050.053.240092
CAACGCA33150.051.4711385
AACGCAG34100.050.0371976
CGCAGAG36950.045.982928
GGTATCA15100.043.8858031
GCAGAGT40600.042.203649
CAGCGCT5650.039.5013779
ACGCAGA44150.038.647077
TATCCCG1251.3273328E-534.5573355
TACATGG33250.031.830552
GTACATG33950.031.1882271
GTGTAGA3500.030.869981
ACATGGG34850.029.9559883
TTATTGA17250.028.381694
CATGGGG22100.028.3429744
CAGGGCA9050.027.84444
CAGAGTA39100.027.8034829
Produced by FastQC (version 0.11.5)