FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115619
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTATATATCACCAACACAGGGGCCTGGTCTGGCTTCTGCTGGTACC54450.488069851804245No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT36270.3251109921935715No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA32150.28818082158873237No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC29070.26057282997152253No Hit
GTATAATCATCAAAGGTCAATCCAGAGGCTACACAGGAGAGTCTCAGGGA23680.21225884464140538No Hit
TGATTATACCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGCCTGGAGT22910.2053568467371029No Hit
TATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTC22530.2019506659531614No Hit
ATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACAGGG22090.19800666715070286No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATGAGTACCA21560.19325594132046872No Hit
GTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG20870.18707103410752238No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAGCGTGA20130.18043794521247844No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19180.17192249325262476No Hit
TTGTATTACTGTGCAAAAGAAGAGGGCGTCTGGGGCCAAGGGACCACGGT18750.16806813078658575No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18080.1620624962464784No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG18070.1619728599100589No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18050.16179358723721987No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17990.1612557692187028No Hit
ATAATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA17680.15847704278969793No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA17070.15300922626810765No Hit
GACTATTACTGTCAATCATCAGACAGAAGTGGTACTCATTGGGTGTTCGG16650.14924450013848814No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16550.148348136774293No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16290.14601759202738568No Hit
CACCAACACAGGGGCCTGGTCTGGCTTCTGCTGGTACCAATAAGCAAATT16180.14503159232677104No Hit
GCCTTGTATTACTGTGCAAAAGAAGAGGGCGTCTGGGGCCAAGGGACCAC15960.1430595929255418No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15260.13678504937617592No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC15110.13544050432988322No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG15080.1351715953206247No Hit
CCCAATGAGTACCACTTCTGTCTGATGATTGACAGTAATAGTCAGCCTCG14880.13337886859223447No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14830.1329306869101369No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14470.12970377879903444No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14120.12656650702435152No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13950.1250426893052198No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA13720.122981053567571No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13590.12181578119411735No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGGCAAAG13240.1186785094194344No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13130.11769250971881977No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12940.11598941932684904No Hit
GTCTGAGAGCTGAGGACACGGCCTTGTATTACTGTGCAAAAGAAGAGGGC12840.11509305596265393No Hit
CTTTATATATCACCAACACAGGGGCCTGGTCTGGCTTCTGCTGGTACCAA12700.11383814725278074No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12700.11383814725278074No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG12680.11365887457994173No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12660.11347960190710268No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12600.11294178388858561No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTAT12420.1113283298330344No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG12390.11105942082377586No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12350.1107008754780978No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12300.11025269379600025No Hit
TATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGC12300.11025269379600025No Hit
CAGTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATA12260.10989414845032221No Hit
GTATTACTGTGCAAAAGAAGAGGGCGTCTGGGGCCAAGGGACCACGGTCA12120.10863923974044902No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12020.10774287637625389No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11980.10738433103057585No Hit
GCCTATTATACCACTTTTCCAACTAATACCTGAGACCCACTCCAGGCCCT11950.10711542202131731No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11890.10657760400280024No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11850.1062190586571222No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11720.10505378628366854No Hit
CTATTACTGTCAATCATCAGACAGAAGTGGTACTCATTGGGTGTTCGGCG11430.10245433252750266No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11410.10227505985466365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)