FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745603
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11290.15142106456116727No Hit
TTGCTACACACTCCTGCTGCTGACCACCCCTTCCTGGGTCTTGTCCCAGG11230.150616346768991No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10990.14739747560028593No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA10450.14015501547069956No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9700.13009604306849623No Hit
CAGTAATATGTGCCTGTGTCCACAGGGTCCATGTTGGTCATGGTAAGGAC9060.12151238661861608No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8550.11467228538511781No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8530.11440404612105906No Hit
ATATTACTGTGCACGGATCGAAAGGAATGATTATTATAGGGGCTACAATT8430.1130628498007653No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG8370.11225813200858901No Hit
GACCTGCACCGTCTCTGGATTCTCACTCAACAATGGTAGAATGGGTGTGA7680.10300387739856198No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC7500.10058972402203316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11100.042.1738134
ATCAACG11650.040.8037073
CAACGCA12450.037.6007465
AACGCAG12600.036.5692636
CGCAGAG12800.036.5603378
TATCAAC13950.033.564412
CAGCGCT3400.031.7628259
GGCAGCG3800.030.3139977
GTACACG4800.030.008295
TACACGG5100.029.6453066
GTACATG20150.029.3142451
TACATGG20150.029.3142452
GCAGAGT16250.029.2413489
ACATGGG19750.028.445213
GTATAAT5250.027.4416731
ACGCAGA17100.027.3668027
CACACCG1100.00998828926.1890535
GTATGTA7800.024.9349821
ACACGGG5950.024.2002497
ACACTCC9650.023.8742358
Produced by FastQC (version 0.11.5)