FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501005
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCATTATAGTGGCAACACCAACTCCAACCCGTCCCTCAAGGGTCGACT26570.17701473346191385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23950.15955976162637697No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19180.12778105336091486No Hit
GAATATATGTCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15630.10413023274406148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16800.060.776914
ATCAACG16950.059.810853
CAACGCA18250.055.9480555
GTATCAA18650.055.6026841
AACGCAG20100.050.80036
ACGCAGA22250.046.214697
TATCAAC22400.045.5796362
CGCAGAG23250.043.9191478
GTACATG37450.034.9970661
TACATGG38250.033.0835232
TACGCAA900.003778203631.9578115
TATACGG1306.807135E-427.6539522
ACATGGG47750.026.5014613
GTATACG1658.8161396E-526.1866171
CATGGGG27800.025.347834
TATAGTG22700.023.44127
GCAGAGT44300.023.212449
TAGTGGC22250.022.6233319
CTATACC6750.022.3704664
GTATTAG7400.021.4093281
Produced by FastQC (version 0.11.5)