FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776705
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24330.3132463419187465No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG24100.3102851146831809No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21500.2768103720202651No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20280.26110299277074306No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19170.2468118526338829No Hit
ATATTACGCAGACTCCGTGAAGGGCCGATTCACCATTTCCAGAGACAATT18440.23741317488621805No Hit
GCTATATTCCCCTCTCAGACAAAAATAGACAGCCGTGTCGTCAGTTCTCA18150.23367945358920053No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17870.230074481302425No Hit
GCTATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGATGGC17810.22930198724097306No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17520.22556826594395557No Hit
GATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGATTTTCAG17360.2235082817800838No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17040.21938831345234033No Hit
GCGTAATATCTATTACTTCCATCATATGATATAACTGCCATCCACTCCAG16410.21127712580709537No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16140.2078009025305618No Hit
ACCTTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCCCCTCAG15710.2022646950901565No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15610.20097720498773666No Hit
GTACATAGCATAGTCGCTTAAGGTGAATCCGGAGGCTGCACAGGAGAGTC15360.19775847973168706No Hit
GTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGATGGCAGTTA15200.19569849556781532No Hit
CTCCTGGACCCCTGCCCGATTTTCAGGCTCCCTCCTTGGGGGCAAGGCTG14430.18578482177918257No Hit
CTATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTT14110.1816648534514391No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12850.16544247816094915No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12160.15655879645425227No Hit
AAGTAAGGTAGATCAACGGAACTATAACTACTGCTATATTCCCCTCTCAG12130.15617254942352632No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT12050.15514255734159044No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12040.15501380833134845No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGTCAGTGTCAGGGCAG12010.1546275613006225No Hit
ATGCTATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGATG11900.15321132218796069No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT11810.15205258109578282No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11720.15089384000360498No Hit
GAACTATAACTACTGCTATATTCCCCTCTCAGACAAAAATAGACAGCCGT11660.15012134594215307No Hit
GTCCAGGAGTGTTTGTTGCTTGTACTATAAATCAGTGCCCTGGGTGCTTG11540.14857635781924924No Hit
CCATAGTAGATCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACC11490.14793261276803935No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11480.14780386375779736No Hit
GAATATAGCAGTAGTTATAGTTCCGTTGATCTACCTTACTTTGACTACTG11270.1451001345427157No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11040.1421389073071501No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT10860.13982142512279436No Hit
CTCTCAGACTGTGGTGACTCAGGAGCCCTCAGTGACTGTGTCCCCAGGAG10800.13904893106134247No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10780.1387914330408585No Hit
ACATAGCATAGTCGCTTAAGGTGAATCCGGAGGCTGCACAGGAGAGTCTG10730.13814768798964858No Hit
ATCTATTACTTCCATCATATGATATAACTGCCATCCACTCCAGCCCCTTG10720.1380189389794066No Hit
ATATAACTGCCATCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAG10700.13776144095892262No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10680.13750394293843865No Hit
GACTTAGTACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCACCACCA10660.1372464449179547No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT10630.13686019788722872No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG10540.13570145679505088No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10540.13570145679505088No Hit
GTGGTGACTCAGGAGCCCTCAGTGACTGTGTCCCCAGGAGGGACAGTCAC10350.1332552256004532No Hit
TACTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10350.1332552256004532No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC10190.13119524143658146No Hit
CTCCTGTGCAGCCTCCGGATTCACCTTAAGCGACTATGCTATGTACTGGG10040.1292640062829517No Hit
GTAATAGATATTACGCAGACTCCGTGAAGGGCCGATTCACCATTTCCAGA9960.12823401420101582No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA9870.12707527310883798No Hit
TTATAGTTCCGTTGATCTACCTTACTTTGACTACTGGGGCCAGGGAACCC9850.126817775088354No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9840.12668902607811203No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT9730.1252727869654502No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA9660.12437154389375632No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9660.12437154389375632No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9640.12411404587327234No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9600.12359904983230442No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG9530.12269780676061053No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC9460.12179656368891666No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9380.12076657160698077No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC9360.12050907358649679No Hit
TCCTCAGGCTGCACACCTGTCAGTGTCAGGGCAGCCTTGCCCCCAAGGAG9320.11999407754552888No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG9280.11947908150456094No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9260.11922158348407697No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9150.11780534437141514No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9100.11716159932020523No Hit
GACTATTACTGCCTGATCTACTATGGTGGTGCTCGGGTGTTCGGCGGAGG9030.11626035624851133No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9020.11613160723826935No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8940.11510161515633349No Hit
CTACTATGGTGGTGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTAC8890.11445787010512358No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT8850.11394287406415564No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8770.11291288198221976No Hit
GCCTTGGGCTGACTTAGTACGGTCAGCTTGGTCCCTCCGCCGAACACCCG8760.11278413297197779No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA8730.11239788594125183No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATAGCATAGTCGC8700.11201163891052589No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC8560.11020915276713811No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8430.10853541563399231No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8360.10763417256229842No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8290.10673292949060455No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8290.10673292949060455No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8210.10570293740866867No Hit
CCACTGGAGCAGTCACCAGTGACTACTATCCAAACTGGTTCCAGCAGAAA8070.1039004512652809No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7900.10171171809116718No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG7900.10171171809116718No Hit
ATATCATATGATGGAAGTAATAGATATTACGCAGACTCCGTGAAGGGCCG7800.10042422798874735No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT7790.10029547897850534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)