FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247949
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATGCTATGAGTTGGT6420.2589242142537377No Hit
GTATTACTGTCAGCAGTATGGTAGCTCTCCTCAGGTCACCTTCGGCCAAG5940.23956539449644887No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT5500.22181980971893414No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5290.21335032607512028No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC5280.21294701733017676No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5130.20689738615602402No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA5080.20488084243130644No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4950.19963782874704072No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC4950.19963782874704072No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC4830.19479812380771852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.1822955527144695No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4430.17866577400997785No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4410.17785915652009082No Hit
CCGTAAGAGTAGTCCCACCGAACAAAGCAGCTAATACTATTACAGATACG4140.16696982040661587No Hit
CTCTTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT3930.15850033676280204No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGACGGTGAGAGT3770.15204739684370577No Hit
TTTAAAAGGTGTCCAATGTGAGGTGCAGGTGGTGGAGTCTGGGGGAGGCT3580.14438453068977897No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG3550.1431746044549484No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC3450.13914151700551322No Hit
CTATTATTGTACTCGAGATGGTCGTATCTGTAATAGTATTAGCTGCTTTG3350.13510842955607807No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT3280.13228526834147344No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC3260.13147865085158641No Hit
GTCTCCAGGCACGCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT3210.12946210712686884No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3130.1262356371673207No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT3100.12502571093249015No Hit
CAATAATAGACGGCTGTGTCCTCGGTTTGCAGGCTGTTCATCTGCAGATA2990.12058931473811146No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC2990.12058931473811146No Hit
CCCTGAGACTCTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATGCT2940.11857277101339388No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCGTGAGATTCCCAGGAGT2900.11695953603361982No Hit
CTATTACAGATACGACCATCTCGAGTACAATAATAGACGGCTGTGTCCTC2880.11615291854373279No Hit
CTGTAATAGTATTAGCTGCTTTGTTCGGTGGGACTACTCTTACGGTATGG2860.11534630105384575No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT2830.1141363748190152No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA2790.11252313983924113No Hit
CTCCAGGCACGCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC2730.11010328736958004No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA2720.10969997862463651No Hit
GTAATAGTATTAGCTGCTTTGTTCGGTGGGACTACTCTTACGGTATGGAC2710.109296669879693No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC2690.10849005238980597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.10607019992014487No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC2600.10486027368531432No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG2600.10486027368531432No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA2590.10445696494037081No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC2590.10445696494037081No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT2570.10365034745048377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.1020371124707097No Hit
GTCTATTATTGTACTCGAGATGGTCGTATCTGTAATAGTATTAGCTGCTT2520.10163380372576619No Hit
CATCTATGGTGCATCCAAGAGGGCCACTGGCATCCCAGACAGGTTCAGTG2520.10163380372576619No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC2520.10163380372576619No Hit
GTATGGTAGCTCTCCTCAGGTCACCTTCGGCCAAGGGACACGACTGGAGA2510.10123049498082268No Hit
ATAATAGACGGCTGTGTCCTCGGTTTGCAGGCTGTTCATCTGCAGATAGG2510.10123049498082268No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC2510.10123049498082268No Hit
GCTAATACTATTACAGATACGACCATCTCGAGTACAATAATAGACGGCTG2500.10082718623587916No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2500.10082718623587916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5250.074.236651
GGTATCA1950.066.6226351
ACACCGG350.003665346261.7016686
TCAACGC6500.058.7786754
TATCAAC6550.058.3299832
CAACGCA6700.057.0240865
ATCAACG6700.057.0240863
AACGCAG6900.055.293046
GTGCTAG1600.049.6199841
CGCAGAG7800.048.9130748
TAGACTG450.0098504248.0580335
CTAGAGA1900.045.5286644
GCAGAGT8700.043.85319
GCTAGAG1755.638867E-1141.1926043
ACGCAGA9300.041.0238657
GTGTAGA1355.128877E-737.4238241
AGCGCTG1752.5520421E-937.073345
CCTACAC1001.435868E-436.0435263
CTACACC1051.912837E-434.3271684
TTAGGAC2950.034.2108042
Produced by FastQC (version 0.11.5)