FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217642
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC20400.16753692793119818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.1536576432153293No Hit
ACCTAGGACGGTGACCTTGGTCCCCGTTCCAAAGACATAAGAACTCAGGC18250.14987984974237092No Hit
GACCTAGGACGGTGACCTTGGTCCCCGTTCCAAAGACATAAGAACTCAGG17850.1465948119397984No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT17410.14298127035696864No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16880.13862859526856006No Hit
CTCCTGCACTGGGAGCAGTTCCAACATCGGGGCAGGTTACGATGTGCACT16730.13739670609259536No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA15030.12343529543166218No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT14860.12203915436556885No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14470.11883624250806067No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12340.10134341620936203No Hit
TCCAGGGACAGCCCCCAAACTCCTCATCTATTCTAACAACAATCGGCCCT12250.1006042827037832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29000.071.7310941
ATCAACG32350.063.2041853
TCAACGC33550.060.9435274
CAACGCA34050.060.0486155
TATCAAC34250.059.7593152
GGTATCA12450.056.8551331
AACGCAG35950.056.6747136
ACGCAGA38900.052.191687
CGCAGAG40700.049.883458
GCAGAGT47800.042.4739849
GTACATG33050.024.4770741
TACATGG34850.023.1613082
GAATACG3453.2159733E-922.9548979
CAGAGTA46600.022.7108429
ACCGACC2603.4055702E-622.1522799
GTGCTAG6050.021.4896851
ACATGGG35500.020.8886623
TAGTACG2557.343324E-519.7633084
GTATTAC4756.1354513E-918.2473831
GAGTACT24650.018.2250810-14
Produced by FastQC (version 0.11.5)