FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217642
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC15430.1267203332342347No Hit
CTCCTGCACTGGGAGCAGTTCCAACATCGGGGCAGGTTACGATGTGCACT14440.11858986467286772No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT14310.11752222738703165No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT13200.10840624748489293No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12610.10356081672609847No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12250.1006042827037832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18750.059.8922464
ATCAACG18800.058.2013443
GTATCAA19200.058.1852761
TATCAAC21550.051.4423372
CAACGCA22200.050.5846635
AACGCAG24000.047.990586
GGTATCA9550.047.546641
ACGCAGA24050.047.292187
CGCAGAG25500.044.3207138
GCAGAGT31450.035.7068259
ACCGACC2900.032.269538
GTACATG33700.027.8032841
TACATGG33900.026.1187692
GTGCTAG6500.024.3945031
TATACTG7750.024.15015
ACATGGG36950.023.378363
GTCTAGC2202.3330615E-522.9328481
CCGACCG4007.548806E-1021.595769
TATACCG1700.003223505621.1723165
CTAGTAC2404.6098852E-520.995883
Produced by FastQC (version 0.11.5)