FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799214
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAGGTCTGGGGGTTATGGTTGGGACTACTGGGGCCA35270.44130858568543596No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGGCTGTGCATTTCCAGATACA24520.3068014324073402No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21530.2693896753560373No Hit
GCTGTATATTACTGTGCGAGGTCTGGGGGTTATGGTTGGGACTACTGGGG17130.21433558471197953No Hit
CCTCTAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGC16120.20169816845050262No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16040.200697184984247No Hit
ATATTACTGTGCGAGGTCTGGGGGTTATGGTTGGGACTACTGGGGCCAGG15710.1965681281859427No Hit
ATATAAAGCCAGATGGAAGTGACACCTATTATGTCGACTCTGTGAAGGGC15110.1890607521890257No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15030.18805976872277014No Hit
GTCTTCTACAGCTCCGACCATCAGAACTACGTCTCTTGGTACCAGCAGAG14950.1870587852565145No Hit
GTCTGGGGGTTATGGTTGGGACTACTGGGGCCAGGGAACCCTGGTCCCCG13420.16791497646437625No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13080.16366079673278996No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12910.16153370686699683No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12860.16090809220058708No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12650.15828051060166615No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12560.1571544042021286No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12500.15640366660243687No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12440.1556529290027452No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12040.1506480116714672No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11740.14689432367300873No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11250.1407632999421932No Hit
GTTTATTACTGTCAACAATATTTTACTACTCCTCTAACGTTCGGCCAAGG11060.13838596420983615No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC11000.13763522661014446No Hit
GCCTGAGAGTCGAGGACACGGCTGTATATTACTGTGCGAGGTCTGGGGGT11000.13763522661014446No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10930.13675936607717082No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10780.13488252207794157No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGGAAATGCACAGCCTGA10480.13112883407948311No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10380.1298776047466636No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10280.1286263754138441No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10110.12649928554805095No Hit
ATATACAGCCGTGTCCTCGACTCTCAGGCTGTGCATTTCCAGATACAGTG10060.12587367088164123No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9940.12437219568225781No Hit
GACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCA9760.12211998288318272No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTTAATAATTATTGGATGACTTGGA9690.12124412235020908No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9600.12011801595067154No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9490.11874166368457009No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9470.11849141781800619No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9460.11836629488472424No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9390.11749043435175059No Hit
ACTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGAGACGTAGTTCTGA9250.11573871328580332No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9000.11261063995375456No Hit
GTAATAAACTGCCACATCTGCAGCCTGCAGGTTGCTGATGGTGAGAGTGA8820.11035842715467947No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8820.11035842715467947No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCT8800.11010818128811557No Hit
GTATCTGGAAATGCACAGCCTGAGAGTCGAGGACACGGCTGTATATTACT8750.10948256662170583No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8730.10923232075514193No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8380.1048530180902737No Hit
CTATTATGTCGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG8330.10422740342386394No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8270.10347666582417225No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8250.10322641995760835No Hit
CCCCCAGACCTCGCACAGTAATATACAGCCGTGTCCTCGACTCTCAGGCT8210.10272592822448054No Hit
GTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACT8160.10210031355807081No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA8120.101599821824943No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)