FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799214
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAGGTCTGGGGGTTATGGTTGGGACTACTGGGGCCA31710.39676482143706193No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGGCTGTGCATTTCCAGATACA22180.27752266601936404No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18520.2317276724381705No Hit
GCTGTATATTACTGTGCGAGGTCTGGGGGTTATGGTTGGGACTACTGGGG13590.17004206633016938No Hit
CCTCTAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGC13560.16966669753032354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13100.16391104259935388No Hit
ATATTACTGTGCGAGGTCTGGGGGTTATGGTTGGGACTACTGGGGCCAGG12440.1556529290027452No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12280.15365096207023402No Hit
GTAATAAACTGCCACATCTGCAGCCTGCAGGTTGCTGATGGTGAGAGTGA12130.15177411807100477No Hit
ATATAAAGCCAGATGGAAGTGACACCTATTATGTCGACTCTGTGAAGGGC12050.15077313460474917No Hit
GTCTTCTACAGCTCCGACCATCAGAACTACGTCTCTTGGTACCAGCAGAG11850.14827067593911017No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA11250.1407632999421932No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10580.13238006341230257No Hit
GTTTATTACTGTCAACAATATTTTACTACTCCTCTAACGTTCGGCCAAGG10430.13050321941307336No Hit
GTCTGGGGGTTATGGTTGGGACTACTGGGGCCAGGGAACCCTGGTCCCCG10360.1296273588800997No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10080.1261239167482051No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9970.12474756448210367No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9840.12312096634943831No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9840.12312096634943831No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9630.1204933847505174No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGGAAATGCACAGCCTGA9600.12011801595067154No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9590.11999289301738959No Hit
ATATACAGCCGTGTCCTCGACTCTCAGGCTGTGCATTTCCAGATACAGTG9200.11511309861939355No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG9080.11361162342001016No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8950.11198502528734483No Hit
GCCTGAGAGTCGAGGACACGGCTGTATATTACTGTGCGAGGTCTGGGGGT8860.11085891888780727No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8820.11035842715467947No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8590.10748059968919463No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8580.1073554767559127No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8490.10622937035637516No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTTAATAATTATTGGATGACTTGGA8480.1061042474230932No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8470.10597912448981124No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8420.10535350982340148No Hit
ACTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGAGACGTAGTTCTGA8100.10134957595837911No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)