FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095755
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA44450.405656373915702No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT30250.27606536132620885No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29570.26985959452614866No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27070.2470442754082801No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20460.18672057166063583No Hit
GTATATTTCTGTGCGAGAGTGAGGCAGTTCGGTGACTACGCGTTGTCCTG20130.18370894953707717No Hit
CAGTATAATAGTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG19130.1745828218899298No Hit
CTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCA19080.1741265155075724No Hit
GTCTGCAACTGTAGGCGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA18260.16664309083691153No Hit
GCTCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCG18090.16509164913689647No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17940.16372272998982437No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC17690.16144119807803753No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17520.15988975637802244No Hit
ATACATTGGGTGCGCCAGGCCCCCGGACAAGGGTTTGAGTGGCTGGGATG17510.15979849510155097No Hit
CTTCCTACATGGAGCTGACCAGCCTGAGATCTGAAGACACGGCTGTATAT17320.15806453084859298No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA17170.15669561170152088No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16830.15359272830149076No Hit
GAAATATACAGCCGTGTCTTCAGATCTCAGGCTGGTCAGCTCCATGTAGG16510.15067236745440357No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16360.14930344830733147No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16270.1484820968190882No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGTTCTGGGAC16200.1478432678837879No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16010.14610930363082988No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15970.145744258524944No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15930.1453792134190581No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15350.1400860593837126No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15220.13889966278958346No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14000.1277657870600636No Hit
ATGCTATACATTGGGTGCGCCAGGCCCCCGGACAAGGGTTTGAGTGGCTG13550.12365902961884727No Hit
GCTGTATATTTCTGTGCGAGAGTGAGGCAGTTCGGTGACTACGCGTTGTC13060.11918722707174505No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12940.11809209175408736No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12400.11316398282462777No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12180.11115623474225533No Hit
GTCCTGGGGCCAGGGGACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGG12140.11079118963636944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.10987857687165471No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12000.1095135317657688No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11780.10750578368339637No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11780.10750578368339637No Hit
GCTATACATTGGGTGCGCCAGGCCCCCGGACAAGGGTTTGAGTGGCTGGG11450.10449416155983773No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11440.10440290028336627No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11390.1039465939010089No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11320.10330776496570858No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA11190.1021213683715794No Hit
TTCTAAAGTAGACGCCTTATAGAGCAGGAGCCTAGGGGCTTTCCCTGGTT10970.10011362028920698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22150.070.6745761
TCAACGC25400.060.4181184
ATCAACG25950.059.1375853
CAACGCA26750.057.6356855
TATCAAC26800.057.261952
AACGCAG28700.054.4130946
CGCAGAG32900.046.8083888
ACGCAGA36850.041.9862177
CAGCGCT5400.031.9832979
GTACACG10150.031.940965
GTACATG32600.031.6443121
TACCTCG1204.2108956E-430.0185875
GCAGAGT53500.029.7265329
ACATGGG33700.029.2895013
TACACGG11550.028.0385866
TATACCG1050.00794945427.4455665
ACACGGG11800.027.4432987
TATGTAC12800.026.4550882
CAGAGTA37800.026.2719969
GTGTAGG5250.026.1078761
Produced by FastQC (version 0.11.5)