FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095755
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA42900.3915108760626235No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT30290.27643040643209477No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28540.26045968304958683No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23780.2170193154491652No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18370.16764696487809777No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17890.16326642360746701No Hit
GTATATTTCTGTGCGAGAGTGAGGCAGTTCGGTGACTACGCGTTGTCCTG17770.1621712882898093No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC17610.16071110786626572No Hit
CAGTATAATAGTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG17080.1558742602132776No Hit
CTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCA16890.1541402959603196No Hit
GCTCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCG16390.1495772321367459No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA16230.1481170517132023No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15990.14592678107788692No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15920.14528795214258663No Hit
GTCTGCAACTGTAGGCGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA15610.1424588525719709No Hit
GAAATATACAGCCGTGTCTTCAGATCTCAGGCTGGTCAGCTCCATGTAGG15400.14054236576606996No Hit
ATACATTGGGTGCGCCAGGCCCCCGGACAAGGGTTTGAGTGGCTGGGATG15290.13953849172488375No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14900.13597930194249627No Hit
ACTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGTTCTGGGAC14650.13369777003070943No Hit
CTTCCTACATGGAGCTGACCAGCCTGAGATCTGAAGACACGGCTGTATAT14420.13159876067186552No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14210.12968227386596456No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14030.12803957088947804No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13640.12448038110709053No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13080.119369749624688No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13010.11873092068938769No Hit
GTCCTGGGGCCAGGGGACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGG12930.11800083047761589No Hit
ATGCTATACATTGGGTGCGCCAGGCCCCCGGACAAGGGTTTGAGTGGCTG12280.11206884750697008No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12250.11179506367755565No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11980.10933100921282587No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11830.10796209006575376No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11440.10440290028336627No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11070.10102623305392174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11030.10066118794803583No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11020.10056992667156436No Hit
GCTATACATTGGGTGCGCCAGGCCCCCGGACAAGGGTTTGAGTGGCTGGG10970.10011362028920698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)