FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090287
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACC31700.2907491330264416No Hit
GTCTACATGGAGATGGGCAGCCTCAGATCTGAGGACACGGCCGTTTATTA31480.28873131569944427No Hit
CTCCTATAGTGGTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCC30720.2817606740243624No Hit
GTAATAAACGGCCGTGTCCTCAGATCTGAGGCTGCCCATCTCCATGTAGA21370.19600343762697345No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT19690.18059465076626613No Hit
CCTATAGTGGTGCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA19560.17940230416394948No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18410.1688546226819177No Hit
CCATGTAGACTGTGCTCGTGGACGTGTCCGCGGTCATGGTGACTCTGCCC17870.1639017983338332No Hit
GTTTATTACTGTGCGAGAGGCTTGGGGCCAGTGGGGGCTACCACCAACTT17720.16252601379269863No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC17020.15610568593407056No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT16530.15161145643303092No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16380.15023567189189635No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16340.14986879601426045No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16100.14766754074844513No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16090.14757582177903616No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA15950.14629175620731055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.14335674918622346No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15540.14253127846154268No Hit
CCACTATAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGC15310.14042174216513634No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAG14960.1372115782358223No Hit
CTCCTGGGCTCCTGCCCGGTTCTCGGGCTCCCTCCTTGGGGGCAAAGCTG14600.13390969533709932No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14380.13189187801010194No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14240.1306078124383763No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14210.13033265553014942No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA13570.12446264148797519No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACCACTA13550.12427920354915722No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13470.12354545179388546No Hit
GATTTATGATACAAGCAACAGACACTCCTGGGCTCCTGCCCGGTTCTCGG13100.1201518499257535No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG13050.11969325507870862No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTATAGTGGTGCTT13000.11923466023166376No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC12990.1191429412622548No Hit
GGATTAATCAACCCTAGTGGTGGCAGTAGACGCTACGCACAGAAGTTCCA12980.11905122229284584No Hit
GTATTACTGCTTGCTCTCCTATAGTGGTGCTTATGTCTTCGGAACTGGGA12870.11804231362934714No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT12330.11308948928126265No Hit
GGGTGCGACAGGCCCCTGGACAAGGGGTTGAGTGGATGGGATTAATCAAC12210.11198886164835499No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12050.11052135813781142No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGCACCACTAT11990.1099710443213576No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11520.1056602527591359No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG11460.10510993894268207No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11140.10217493192159496No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT11000.10089086634986935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.074.925071
GGTATCA8850.065.996321
ATCAACG25200.063.7475363
TCAACGC25550.062.8742834
CAACGCA25800.062.2650345
AACGCAG26600.060.6047976
TATCAAC27000.060.298122
CGCAGAG31850.050.6126758
ACGCAGA33000.048.8488967
GTACACG8100.036.4634635
GTTTACG850.002843506633.8658339
TACACGG9100.032.4252326
GTACATG31000.031.6340791
ACACGGG9400.031.3889457
TAAACGG13550.031.3669175
ATAAACG14150.031.0550694
ACATGGG30050.030.9246183
GCAGAGT54650.029.7604149
TACGCAA1000.00629855828.785967
AACGGCC15100.028.1187367
Produced by FastQC (version 0.11.5)