FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021420
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGCAGTGCCATTTGGGTGCTCGGCGGGGGGACCAAGGTGACCG42630.41736014567954416No Hit
GCCCATATATAGCATACAGTAATAATCAGACTCATCATCTGCCTGGGCCC41170.4030663194376456No Hit
ATATAGCATACAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGA30740.3009535744355897No Hit
GTAATATTCAGCCATGTCCTCACGTCTCAGGCTGTCCATTTGAAGATACA26030.2548412993675471No Hit
GTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG25180.2465195512130172No Hit
GAATATTACTGTGCGAGGGGCAACTCCGTGCTCCACTACTACCACGGTTT23400.22909283154823679No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21660.21205772356131658No Hit
ATGCTATATATGGGCAGTGCCATTTGGGTGCTCGGCGGGGGGACCAAGGT21450.21000176225255038No Hit
CCCATATATAGCATACAGTAATAATCAGACTCATCATCTGCCTGGGCCCC20650.2021695286953457No Hit
GATTATTACTGTATGCTATATATGGGCAGTGCCATTTGGGTGCTCGGCGG17970.17593154627871002No Hit
GTGGTGACCCAGGAGCCATCGCTCTCAGTGTCCCCTGGAGGGACAGTCAC17000.16643496309059935No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15780.15449080691586223No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15320.14998727262046954No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15210.1489103405063539No Hit
TATTAATAATGATGGCGACCGCAGATATTATGCAGATTTTGTGAAGGGCA15010.14695228211705272No Hit
CCCTAAGACTCTCTTGTGCAGCCTCTGGATTCATCTTCAGTAATTATGCT14550.14244874782166003No Hit
TTTTAAAGGTGTCCAGTGTGAGGTGCAGCTGCTGGAGTCTGGGGGAGACT14500.14195923322433476No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA14080.1378473106068023No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13590.13305006755301443No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13590.13305006755301443No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13400.13118991208317832No Hit
ATATTACTGTGCGAGGGGCAACTCCGTGCTCCACTACTACCACGGTTTGG13370.13089620332478313No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13350.13070039748585302No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13330.1305045916469229No Hit
CATTTATAGCACAAATACTCGATCTTCTGGTGTCCCTGATCGATTCTCTG13210.12932975661334223No Hit
GACCTAGGACGGTCACCTTGGTCCCCCCGCCGAGCACCCAAATGGCACTG13070.12795911574083138No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGCTCTCAGTGTCCCCTGGAG12730.1246304164790194No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12570.12306396976757847No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12520.12257445517025317No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12270.12012688218362672No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11980.11728769751914003No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11800.11552544496876897No Hit
CAGTAATAATCAGACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC11720.11474222161304849No Hit
TTCTTGTAGTTGTCTCTGGAGATGGTGAATCTGCCCTTCACAAAATCTGC11680.11435060993518827No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11600.1135673865794678No Hit
TTATTAATATTTGCGACATATTCAAGTCCCCTCCCTGGAGCCCGGCGGAC11560.11317577490160757No Hit
CTACAAGAATATGGTGTATCTTCAAATGGACAGCCTGAGACGTGAGGACA11500.11258835738481722No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11450.11209884278749191No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11390.11151142527070157No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11230.10994497855926064No Hit
ATAAATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG11150.10916175520354018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.108868046445145No Hit
CTCATTTATAGCACAAATACTCGATCTTCTGGTGTCCCTGATCGATTCTC11110.10877014352567993No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11080.10847643476728476No Hit
GCCTGAGACGTGAGGACATGGCTGAATATTACTGTGCGAGGGGCAACTCC10880.1065183763779836No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10840.10612676470012335No Hit
GTCATAACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGC10780.10553934718333302No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10640.10416870631082219No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10580.10358128879403183No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10550.10328758003563666No Hit
GAACACAGAAGAGTCATGACGGAGTTTGGGCTGAGTTGGGTTTTCCTTGT10530.10309177419670654No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA10450.10230855084098608No Hit
CAGTAATATTCAGCCATGTCCTCACGTCTCAGGCTGTCCATTTGAAGATA10360.10142742456580056No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10350.10132952164633548No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)