FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281694
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT44350.34602643064569233No Hit
GTATGATAATCTCCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA39360.3070935808391082No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35930.2803321229560254No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA35210.274714557452871No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCT31750.24771903434049003No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31750.24771903434049003No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC29540.23047622911552992No Hit
ATATTACTGTCAACAGTATGATAATCTCCCTTTCACTTTCGGCCCTGGGA26850.20948838022180022No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG25240.19692687958280214No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21080.1644698344534655No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA20880.16290939959147815No Hit
ACATATTACTGTCAACAGTATGATAATCTCCCTTTCACTTTCGGCCCTGG20790.16220720390358387No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA20390.15908633417960918No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20100.15682370362972753No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19450.15175229032826867No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19400.15136218161277185No Hit
CTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA19350.15097207289727502No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18770.14644681179751173No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18260.14246770289944402No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17960.14012705060646302No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA17840.13919078968927062No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17770.13864463748757505No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17130.13365124592921554No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17040.13294905024132125No Hit
GTGTATTACTGTGCGAGACATTACTATGATGGCCCCTACGGTATGGACGT16640.12982818051734657No Hit
ACTGTCAACAGTATGATAATCTCCCTTTCACTTTCGGCCCTGGGACCAAA16190.12631720207787506No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGT15820.12343039758319849No Hit
TCATAGTAATGTCTCGCACAGTAATACACGGCCGTGTCTGCGGCGGTCAC15730.1227282018953042No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15680.12233809317980734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15330.1196073321713295No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14910.1163304189611561No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14820.11562822327326179No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14390.11227328831998902No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14200.11079087520110105No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14120.11016670125630611No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13910.10852824465121941No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13520.1054853966703441No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13420.10470517923935041No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTCCCTT13400.1045491357531517No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13340.1040810052945555No Hit
GATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGC13270.10353485309285992No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC13040.1017403530015745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19250.079.346641
TCAACGC22600.066.26854
ATCAACG22700.065.979153
CAACGCA23200.064.552155
AACGCAG24300.061.6300356
TATCAAC24750.060.514212
CGCAGAG27600.054.778698
GCAGAGT32850.045.8049439
ACGCAGA34450.043.886567
GTACATG27950.035.3152471
TACATGG29100.033.157442
TACGTAC900.003752839832.0014574
TAGCGTA900.00375499131.9977117
ACATGGG29750.031.7068823
CAGCGCT6000.029.9978549
GTATAAC2252.994966E-828.8193021
TCTATTA27200.028.0611952
ATCTATT27700.027.5708121
CTATTAC28850.027.205073
CAGAGTA33100.026.5358649
Produced by FastQC (version 0.11.5)