FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281694
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT42260.32971988633792465No Hit
GTATGATAATCTCCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCA39710.30982434184758606No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36390.2839211231385963No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA36010.28095629690082036No Hit
TTATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCT32250.25162012149545837No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29730.2319586422344179No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC28130.21947516333851919No Hit
ATATTACTGTCAACAGTATGATAATCTCCCTTTCACTTTCGGCCCTGGGA26600.20753783664431602No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA26040.2031686190307515No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG25430.1984092927016901No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20950.16345555179317373No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA20760.16197313867428575No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20220.15775996454691993No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19960.1557313992263364No Hit
ACATATTACTGTCAACAGTATGATAATCTCCCTTTCACTTTCGGCCCTGG19560.1526105295023617No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18990.1481632901456978No Hit
CTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA18690.1458226378527168No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18590.14504242042172313No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA18340.14309187684423896No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17930.1398929853771649No Hit
GTGTATTACTGTGCGAGACATTACTATGATGGCCCCTACGGTATGGACGT17850.13926881143237No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17120.13357322418611617No Hit
TCATAGTAATGTCTCGCACAGTAATACACGGCCGTGTCTGCGGCGGTCAC16800.13107652840693643No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16530.12896994134325354No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16460.12842378914155797No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15970.124600723729689No Hit
ACTGTCAACAGTATGATAATCTCCCTTTCACTTTCGGCCCTGGGACCAAA15770.12304028886770166No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15750.12288424538150292No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGT15530.12116776703331684No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15300.1193732669420314No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14780.11531613630086433No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14630.11414581015437382No Hit
GATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGATCCAGC14320.11172713611829345No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13690.10681176630303332No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13640.1064216575875365No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13580.1059535271289403No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG13550.10571946189964218No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC13500.10532935318414535No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13330.10400298355145612No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13040.1017403530015745No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT12970.10119420079987893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16150.065.1264651
TCAACGC16150.064.662724
ATCAACG16950.061.6131973
CAACGCA17050.061.2470445
AACGCAG18500.056.424576
TATCAAC19700.053.3821372
CGCAGAG20800.050.877538
GCAGAGT24900.042.5001039
ACGCAGA28900.036.1195347
TTACGGT800.00210420136.0104454
TATACCG800.002104604236.0090375
GTACATG31450.030.0073621
GTATAGG4100.029.8704811
TACGACG1453.6537356E-529.8005855
CAGCGCT6600.029.4504419
GGCAGCG6850.028.375617
ACATGGG32400.028.231513
TACATGG32750.028.151922
GTCCGGT1050.00795212227.4440291
GCAGCGC7600.026.5226218
Produced by FastQC (version 0.11.5)