FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882637
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC24280.27508477437496953No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCCAGG20670.23418460816847694No Hit
GATGTACACTGGTACCAGCAGTTTCCAGGAACAGCCCCCAAACTCCTCAT19270.21832304786679008No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA15030.17028517952453842No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG15020.17017188266524064No Hit
GGTTATGATGTACACTGGTACCAGCAGTTTCCAGGAACAGCCCCCAAACT14550.16484693027824576No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT14250.16144802449931286No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13170.1492119636951544No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13130.14875877625796335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.14807899510217679No Hit
TCCTATGACAGAAGCCTGGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCT12260.13890194949905793No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCCAGGC11960.13550304372012503No Hit
CTTCTATACTCCGTGAATCTCGAACAGTAATACACGGCCGTGTCTGCGGC11570.13108446620751227No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG11550.13085787248891673No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11490.13017809133313016No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10900.12349357663456212No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10800.12236060804158447No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10690.12111434258930907No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT10550.1195281865591404No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAACTGGATCCG10540.11941488969984264No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10340.11714895251388735No Hit
TCTATACTCCGTGAATCTCGAACAGTAATACACGGCCGTGTCTGCGGCGG10270.11635587449880301No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10090.11431653103144328No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10070.11408993731284775No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10020.11352345301635894No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9670.10955806294093721No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9290.10525278228762219No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9250.10479959485043115No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9240.1046862979911334No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9190.10411981369464457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8700.069.6856161
GTATCAA21900.066.901371
ATCAACG22550.064.172463
TCAACGC22950.063.0539864
TATCAAC24050.059.6354982
CAACGCA24700.058.8780755
AACGCAG25850.056.258746
ACGCAGA26600.053.860537
CGCAGAG27600.051.6482128
GCAGAGT31650.044.5842559
GAATACG4300.038.5086759
GTGTAGA6450.035.8074461
ACATGGG22650.032.4213523
GTACATG23550.031.8731881
TACATGG23650.031.6934192
GTGCTAG5600.029.643051
ACCGACC1804.9620176E-627.9978548
GTCTACG1100.00988548526.2452281
GCTAGAG6050.026.179813
CTAGAGA6900.026.0849554
Produced by FastQC (version 0.11.5)