FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501527
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.3052677124063111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.21633929977847655No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10720.2137472160023289No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC10440.20816426633062626No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT10370.20676852891270062No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA9730.19400750109166603No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT9420.18782637824085244No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACATTCCCGAAAGTG8990.17925256267359485No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC8940.1782556073750765No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACATTCCCGAAAGTGC8810.17566352359892887No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8330.16609275273315297No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8220.16389945107641263No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC8110.16170614941967232No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT7980.15911406564352468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.15771832822559903No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGCACTTTCGGGAATGTCTT7810.15572441762856237No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT7610.15173659643448908No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT7240.14435912722545347No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7140.1423652166284168No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG6780.1351871384790849No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA6720.1339907921208629No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6300.12561636761330897No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT6160.12282489277745764No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT5860.1168431609863477No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC5730.11425107721020004No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACATT5530.11026325601612676No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT5510.10986447389671943No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG5440.10846873647879376No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC5270.10507908846383146No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA5270.10507908846383146No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATA5110.10188883150857282No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT5100.10168944044886916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.096.094031
GTATCAA16500.075.285111
TATCAAC21650.057.052482
TCAACGC21650.056.4041564
ATCAACG22000.055.825823
CAACGCA22350.054.6375855
AACGCAG22500.054.2733356
GAATACG1204.820322E-946.787369
ACGCAGA26150.046.6978957
CGCAGAG26700.045.7359548
GTGCTAG1705.4569682E-1141.282961
GCAGAGT30050.040.870829
ACGCCGG750.001227614240.1799125-129
GTACATG18900.034.9048541
TACATGG18550.034.8067632
GCTAATA1959.580617E-932.3912432
CATGGGG13300.032.1882934
CAGAGTA29000.032.1864749
ACATGGG19650.032.1439863
AGACGGC3150.031.191576
Produced by FastQC (version 0.11.5)