FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501527
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT10760.21454478024114354No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9840.19620080274840634No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC9240.18423733916618645No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT9140.18224342856914982No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC9110.18164525539003884No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA8340.16629214379285662No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT8240.16429823319581996No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACATTCCCGAAAGTG8180.16310188683759796No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT7730.15412928915093305No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC7450.14854633947923043No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC7410.14774877524041577No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACATTCCCGAAAGTGC7280.14515669146426813No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGCACTTTCGGGAATGTCTT7240.14435912722545347No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT7230.14415973616574979No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA6920.1379786133149362No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6840.13638348483730686No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6800.1355859205984922No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6770.1349877474193812No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG6430.1282084513894566No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT6070.12103037324012467No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6040.12043220006101366No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT6020.12003341794160634No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT5610.11185838449375607No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA5410.10787056329968275No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA5360.10687360800116445No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTGCCGAAGACATT5080.10129065832946181No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT5040.10049309409064716No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG5030.10029370303094351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.088.310651
GTATCAA10050.061.48111
TCAACGC12050.050.6940774
ATCAACG12150.050.276843
CAACGCA12600.048.481245
TATCAAC12800.047.7237172
AACGCAG13100.046.630816
ACGCAGA15550.039.2838337
GTACATG19000.038.063491
CGCAGAG16050.038.0600368
TACATGG19700.037.780232
TAGACGG2550.035.795485
CATGGGG13400.035.6310924
ACATGGG21600.034.456973
GGCGGGG4950.033.5427145
CACGGTA1352.6639493E-531.2063122
GCAGAGT19200.031.0844159
AGACGGC3250.030.246126
ATAGACG3051.8189894E-1229.9273664
TCTACAC2601.6007107E-1029.706013
Produced by FastQC (version 0.11.5)