FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773092
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATATGTGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATA17770.22985621374946322No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14720.19040424684254914No Hit
CTATGGCACTGCTTCTGATCGTGTCTGCTGGGGCCAGGGATCCCTGGTCA14080.1821258013276557No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13840.17902138425957068No Hit
ATATTATTGTGTGAAAGCCGGGGGACACTATGGCACTGCTTCTGATCGTG13650.17656372074733667No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC12540.16220579180744335No Hit
GTGCCATAGTGTCCCCCGGCTTTCACACAATAATATGTGGCCGTGTCCTC12270.1587133226058477No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11980.1549621519819116No Hit
GTCCAGAGACAATGCCAAGAGCACCCTGTATTTGCAAATGAACAGCCTGA11820.15289254060318824No Hit
GTGTTAGTGGTGTCACAACGTACCACACAGACTCCCTGAAGGGCCGGTTT11810.15276318989201804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.15198708562499677No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11650.15069357851329468No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11510.14888266855691173No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11260.1456489007776565No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11240.14539019935531605No Hit
ATCAGAAGCAGTGCCATAGTGTCCCCCGGCTTTCACACAATAATATGTGG11030.14267383442074164No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10600.13711175384042262No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10130.13103227041542273No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10040.12986811401489087No Hit
GGACTGAACAGAGAGAACTCGCCATGGAGGTTGGGCTGACCTGGCTTTTT9780.1265049955244654No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9760.12624629410212496No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9530.12327122774521014No Hit
GTACCACACAGACTCCCTGAAGGGCCGGTTTACTCCGTCCAGAGACAATG9380.121330967077657No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9340.12081356423297616No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9250.11964940783244427No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9210.11913200498776341No Hit
GAGTAAACCGGCCCTTCAGGGAGTCTGTGTGGTACGTTGTGACACCACTA9180.1187439528542528No Hit
TCTCTGGACGGAGTAAACCGGCCCTTCAGGGAGTCTGTGTGGTACGTTGT9170.11861460214308257No Hit
ATAATATGTGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA9020.11667434147552944No Hit
GCCTGGGGAGTCCCTGCGACTCTCCTGTGTGGCCTCTGGATTCCCTTTTG8800.1138286258297848No Hit
TTTAAAAGGTGTCCAGTGTGAGATGCAGATGTTGGCGTCTGGGGGAGACT8560.11072420876169976No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8390.10852524667180621No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8350.10800784382712536No Hit
GTGTGGTACGTTGTGACACCACTAACACTAATCCCTGAGACCCACTCCAG8070.10438602391435949No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7990.1033512182249978No Hit
GCTTCTGATCGTGTCTGCTGGGGCCAGGGATCCCTGGTCATCGTCTCCTC7930.10257511395797654No Hit
GATCAGGACTGAACAGAGAGAACTCGCCATGGAGGTTGGGCTGACCTGGC7760.10037615186808298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)