FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773092
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATATGTGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATA17340.22429413316914418No Hit
CTATGGCACTGCTTCTGATCGTGTCTGCTGGGGCCAGGGATCCCTGGTCA15210.19674243168988945No Hit
ATATTATTGTGTGAAAGCCGGGGGACACTATGGCACTGCTTCTGATCGTG13770.1781159292813792No Hit
GTGCCATAGTGTCCCCCGGCTTTCACACAATAATATGTGGCCGTGTCCTC12340.15961877758403914No Hit
GTGTTAGTGGTGTCACAACGTACCACACAGACTCCCTGAAGGGCCGGTTT12120.15677306193829454No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11850.15328059273669886No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11780.1523751377585074No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA11600.15004682495744362No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11570.149658772823933No Hit
GTCCAGAGACAATGCCAAGAGCACCCTGTATTTGCAAATGAACAGCCTGA11390.1473304600228692No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10970.1418977301537204No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10690.1382759102409545No Hit
GGACTGAACAGAGAGAACTCGCCATGGAGGTTGGGCTGACCTGGCTTTTT10480.1355595453063801No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10220.13219642681595464No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10160.13142032254893338No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10090.1305148675707419No Hit
ATCAGAAGCAGTGCCATAGTGTCCCCCGGCTTTCACACAATAATATGTGG10040.12986811401489087No Hit
GAGTAAACCGGCCCTTCAGGGAGTCTGTGTGGTACGTTGTGACACCACTA9720.12572889125744413No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9630.12456473485691225No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9590.1240473320122314No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9590.1240473320122314No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9140.11822655000957194No Hit
GTACCACACAGACTCCCTGAAGGGCCGGTTTACTCCGTCCAGAGACAATG9130.11809719929840173No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG8980.1161569386308486No Hit
GCCTGGGGAGTCCCTGCGACTCTCCTGTGTGGCCTCTGGATTCCCTTTTG8970.11602758791967839No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8810.11395797654095502No Hit
ATAATATGTGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA8800.1138286258297848No Hit
TTTAAAAGGTGTCCAGTGTGAGATGCAGATGTTGGCGTCTGGGGGAGACT8510.11007745520584872No Hit
TCTCTGGACGGAGTAAACCGGCCCTTCAGGGAGTCTGTGTGGTACGTTGT8440.10917200022765726No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8200.10606758315957221No Hit
GATCAGGACTGAACAGAGAGAACTCGCCATGGAGGTTGGGCTGACCTGGC8170.10567953102606158No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8110.10490342675904032No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC8050.10412732249201906No Hit
GCTTCTGATCGTGTCTGCTGGGGCCAGGGATCCCTGGTCATCGTCTCCTC7980.10322186751382759No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7780.1006348532904234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)