FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402215
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT38930.27763217480914126No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28950.20645906654828253No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGA24570.17522277254201388No Hit
GTAATGGAAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT21920.15632410151082396No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21560.15375673488017172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.14962042197523204No Hit
GTCTACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA19600.1397788498910652No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19110.1362843786437886No Hit
ACCTAGGGCCAGTCCAAAAATCGTAACCACCGCTGTGGGCTCTCGCACAG18760.1337883277528767No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18220.12993727780689837No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18180.129652014847937No Hit
CTATATGCACTGGGTGCGACTGGCCCCAGGACAAGGGCTTGAGTGGATGG18070.1288675417107933No Hit
GTGTATTACTGTGCGAGAGCCCACAGCGGTGGTTACGATTTTTGGACTGG16090.11474702524220608No Hit
TCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG15810.11275018452947658No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT15600.11125255399492945No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15330.10932702902194029No Hit
GTCTGGGGCTGAGGTGAAGAGGCCTGGGGCCTCAGTGAAGGTTTCCTGCA15000.10697360961050909No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14610.10419229576063586No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14320.10212413930816601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27000.077.198151
ATCAACG32700.062.5605473
TCAACGC32750.062.4650344
GGTATCA11750.060.7673261
CAACGCA34200.060.027295
TATCAAC34750.059.2845042
AACGCAG35450.057.8590436
CGCAGAG39200.051.9568758
ACGCAGA41250.049.2003067
GTACATG37650.033.5232351
ACATGGG37400.032.1643563
CAGAGTA44750.026.0534119
GCAGAGT84600.025.6058259
ATACACG21150.025.2029464
CATGGGG23450.024.5740724
CCTATAC5150.023.77783
CAGCGCT5800.023.57599
CTATACC7350.023.5208974
TACATGG54250.023.103592
GTAATAC24350.022.8068121
Produced by FastQC (version 0.11.5)