FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587205
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA44430.7566352466344803No Hit
GTATATTACTGTGCGAGAGATTTATCTATGGTCTTTGACTCCTGGGGCCA34970.5955330761829345No Hit
ATATTACGCAGACTCAGTGAAGGGCCGCTTCACCATCTCCAGAGACAACG20120.34264013419504263No Hit
GACATATATTACGCAGACTCAGTGAAGGGCCGCTTCACCATCTCCAGAGA17370.2958081079009886No Hit
ATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAGTG15620.2660059093502269No Hit
GTAATAGTCAACCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG15490.2637920317435989No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14090.2399502729029896No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14030.23892848323839203No Hit
GCTGTATATTACTGTGCGAGAGATTTATCTATGGTCTTTGACTCCTGGGG13790.2348413245800019No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT13560.23092446419904464No Hit
ATATATGTCAGTACTACTAGTAGTAATGGATGAGACCCACTCCAGCCCCT12790.2178114968367095No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT11900.20265495014517929No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11510.19601331732529526No Hit
GCGTAATATATGTCAGTACTACTAGTAGTAATGGATGAGACCCACTCCAG11000.18732810517621615No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10980.1869875086213503No Hit
GACTATTACTGTGCTGCATGGGATGACAGCCTGAATGGGTATGTCTTCGG10840.18460333273728938No Hit
ATATTACTGTGCGAGAGATTTATCTATGGTCTTTGACTCCTGGGGCCACG10620.18085677063376504No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10420.17745080508510658No Hit
GATCAGCACTGAGCACCGAGGATCCACCATGGAACTGGGGCTCCGCTGGG9750.16604082049710067No Hit
GTCCTGGGCCCAGGCTGTGCTGTCTCAGCCACCCTCAGCGTCTGGGACCC9320.15871799456748495No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC9120.1553120290188265No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA8970.15275755485733264No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8950.15241695830246677No Hit
TAGTAGTACTGACATATATTACGCAGACTCAGTGAAGGGCCGCTTCACCA8910.15173576519273507No Hit
CAGTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA8890.15139516863786923No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8840.1505436772507046No Hit
GTGCAGGGTCCTGGGCCCAGGCTGTGCTGTCTCAGCCACCCTCAGCGTCT8630.1469674134246132No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT8620.14679711514718027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.14134757026932673No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT8150.1387930961078329No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGT8090.13777130644323535No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG8090.13777130644323535No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTTTCTGCAAATGAACAGCCTGA8050.13709011333350363No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATAATTATGGCATGAACTGGG7880.13419504261714393No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAATAATTATGGC7830.13334355122997932No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG7730.1316405684556501No Hit
GACCTAGGACGTTGACCTTGGTCCCAGTTCCGAAGACATACCCATTCAGG7700.1311296736233513No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT7620.12976728740388793No Hit
GGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAGCGGGG7500.12772370807469283No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA7400.12602072530036357No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7380.12568012874549775No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC7240.1232959528614368No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTG7160.12193356664197341No Hit
GCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACAC7040.11988998731277833No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG6940.11818700453844909No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCA6870.11699491659641861No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGGCTGTGCTGTCTCAGCCAC6860.11682461831898572No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT6860.11682461831898572No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG6770.11529193382208938No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA6740.11478103898979063No Hit
TTACTGTGCTGCATGGGATGACAGCCTGAATGGGTATGTCTTCGGAACTG6660.11341865277032723No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT6640.11307805621546137No Hit
GTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGA6540.11137507344113214No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCT6440.1096720906668029No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA6200.10558493200841274No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6170.10507403717611395No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG6090.10371165095665058No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG6080.10354135267921766No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC6030.10268986129205304No Hit
GCACTGAGCACCGAGGATCCACCATGGAACTGGGGCTCCGCTGGGTTTTC5970.10166807162745549No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA5900.10047598368542503No Hit
GTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT5880.10013538713055917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)