FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587205
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA44060.7503342103694621No Hit
GTATATTACTGTGCGAGAGATTTATCTATGGTCTTTGACTCCTGGGGCCA35150.598598445176727No Hit
ATATTACGCAGACTCAGTGAAGGGCCGCTTCACCATCTCCAGAGACAACG19550.33293313238136596No Hit
GACATATATTACGCAGACTCAGTGAAGGGCCGCTTCACCATCTCCAGAGA17380.29597840617842147No Hit
ATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAGTG16080.2738396301121414No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT15370.2617484524144038No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT14450.24608101089057485No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA13860.23603341252203233No Hit
GCTGTATATTACTGTGCGAGAGATTTATCTATGGTCTTTGACTCCTGGGG13120.223431339991996No Hit
ATATATGTCAGTACTACTAGTAGTAATGGATGAGACCCACTCCAGCCCCT12580.2142352330106181No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12490.2127025485137218No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10880.18528452584702107No Hit
GTCCTGGGCCCAGGCTGTGCTGTCTCAGCCACCCTCAGCGTCTGGGACCC10840.18460333273728938No Hit
GCGTAATATATGTCAGTACTACTAGTAGTAATGGATGAGACCCACTCCAG10840.18460333273728938No Hit
ATATTACTGTGCGAGAGATTTATCTATGGTCTTTGACTCCTGGGGCCACG10640.1811973671886309No Hit
GACTATTACTGTGCTGCATGGGATGACAGCCTGAATGGGTATGTCTTCGG10430.1776211033625395No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10180.1733636464267164No Hit
GTGCAGGGTCCTGGGCCCAGGCTGTGCTGTCTCAGCCACCCTCAGCGTCT9850.1677438032714299No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT9650.16433783772277144No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9570.16297545150330803No Hit
GTAATAGTCAACCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG9470.1612724687289788No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT9230.15718531007058864No Hit
GATCAGCACTGAGCACCGAGGATCCACCATGGAACTGGGGCTCCGCTGGG9230.15718531007058864No Hit
TAGTAGTACTGACATATATTACGCAGACTCAGTGAAGGGCCGCTTCACCA8860.15088427380557046No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8780.14952188758610707No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA8630.1469674134246132No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8450.14390204443082058No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC8360.14236935993392427No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT8320.14168816682419258No Hit
CAGTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA8260.14066637715959504No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8030.13674951677863778No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8020.13657921850120486No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTTTCTGCAAATGAACAGCCTGA7930.13504653400430855No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGT7850.13368414778484516No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT7820.1331732529525464No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG7790.13266235812024763No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7770.13232176156538178No Hit
GGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAGCGGGG7760.13215146328794883No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGGCTGTGCTGTCTCAGCCAC7740.131810866733083No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAATAATTATGGC7620.12976728740388793No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATAATTATGGCATGAACTGGG7540.12840490118442452No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTG7160.12193356664197341No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG6830.11631372348668693No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG6810.11597312693182107No Hit
GACCTAGGACGTTGACCTTGGTCCCAGTTCCGAAGACATACCCATTCAGG6810.11597312693182107No Hit
GCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACAC6710.11427014415749184No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG6700.11409984588005892No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC6640.11307805621546137No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG6620.11273745966059553No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA6450.10984238894423584No Hit
GTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCT6420.10933149411193706No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT6280.10694731822787613No Hit
TTACTGTGCTGCATGGGATGACAGCCTGAATGGGTATGTCTTCGGAACTG6250.10643642339557735No Hit
GTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGA6240.10626612511814443No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC6110.10405224751151641No Hit
GATCATTACTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGG6050.10303045784691887No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC6040.10286015956948596No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA5990.10200866818232135No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCA5980.10183836990488843No Hit
GTACTACTAGTAGTAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGC5940.1011571767951567No Hit
GTACTGACATATATTACGCAGACTCAGTGAAGGGCCGCTTCACCATCTCC5930.10098687851772381No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA5890.10030568540799209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGCG3200.038.247767
AATATAC17450.036.7324263
GCAGCGC3550.036.5017978
ACAGCCG17700.035.7914668
TCAACGC10550.035.492124
CAGCCGT17600.035.176769
GAATACG1459.184005E-734.7536249
AACGCAG11000.034.6888776
ATCAACG10850.034.516653
CAACGCA11000.034.040175
GTGCTAG1501.1923657E-633.6123431
ATACAGC18650.033.5851636
TATACAG19300.033.5788845
GTATGTA7300.033.5465661
TACAGCC19150.033.4601827
GTATCAA11650.033.3856541
CGCAGAG11500.033.177848
TATCAAC11700.032.627372
TATTGGG3100.032.5280722
GTACATG15500.031.1340121
Produced by FastQC (version 0.11.5)