FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863090
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA37990.4401626713320743No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG34400.39856793613644No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33810.3917320325806115No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT33150.3840850896198542No Hit
ATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA26520.3072680716958834No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26290.3046032279368316No Hit
TTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC23840.27621684876432356No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT22460.26022778621001286No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22030.25524568700830735No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA21230.24597666523769246No Hit
GTACTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAG20930.24250078207371187No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19510.22604826843087045No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG19420.2250055034816763No Hit
GAGTACTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGC18530.21469371676186727No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG17190.1991681052960873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16280.18862459303201287No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG14370.16649480355466983No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT14110.16348237147922No Hit
GTGTATTACTGTGCGAGACCGGATGATTACTACTACTACTACGGTATGGA14030.16255546930215853No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC14010.16232374375789316No Hit
GTGTTACCACTGTTAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCC13840.16035407663163748No Hit
CCGTAGTAGTAGTAGTAATCATCCGGTCTCGCACAGTAATACACGGCCGT13500.15641474237912614No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT13490.15629887960699348No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA13120.1520119570380841No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT11920.13810842438216178No Hit
GTGGTAACACAGGCTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACC11860.13741324774936564No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAA11650.13498012953457925No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11430.13243114854766014No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11350.13150424637059865No Hit
CCCTAACAGTGGTAACACAGGCTATGCACAGAAGTTCCAGGGCAGAGTCA11300.13092493250993523No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11020.12768077489022003No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11010.12756491211808732No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10940.12675387271315852No Hit
CTGTTAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCCAGTGGCCTG10940.12675387271315852No Hit
CATGTAGGCTGTGCTTATGGAGGTGTTCCTGGTCATGGTGACTCTGCCCT10740.12443661727050481No Hit
CCATGTAGGCTGTGCTTATGGAGGTGTTCCTGGTCATGGTGACTCTGCCC10610.1229304012327799No Hit
ATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTG10590.12269867568851453No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10350.11991796915733006No Hit
GCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTTCAGTG10340.11980210638519737No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA10310.11945451806879932No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9800.11354551669003232No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC9690.11227102619657278No Hit
CTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT9630.11157584956377667No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC9560.11076481015884787No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9490.10995377075391907No Hit
GCTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG9360.10844755471619413No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGGGAGT9330.10809996639979608No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9220.10682547590633654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.10566684818500967No Hit
GGATACACCTTCACCAGTTATGATATCAACTGGGTGCGACAGGCCACTGG9120.10566684818500967No Hit
TCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGCT9040.1047399460079482No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG8940.10358131828662133No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA8840.10242269056529446No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8820.10219096502102909No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC8770.10161165116036566No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG8730.10114820007183492No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8680.1005688862111715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)