FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863090
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA39450.45707863606344645No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38310.4438702800403202No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG34400.39856793613644No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT32840.380493343683741No Hit
ATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA25820.2991576776465954No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25070.2904679697366439No Hit
TTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC22570.2615022767034724No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT21900.25373947097058247No Hit
GTACTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAG21860.25327601988205173No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA21370.24759874404755008No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21340.24725115573115206No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG20470.23717109455560834No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC17740.20554055776338506No Hit
GAGTACTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGC17440.20206467459940447No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16830.19499704549931063No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15680.18167282670405172No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14850.17205621661703877No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14460.16753756850386403No Hit
GTGTATTACTGTGCGAGACCGGATGATTACTACTACTACTACGGTATGGA14300.16568376414974106No Hit
GTGTTACCACTGTTAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCC14230.16487272474481227No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG13690.1586161350496472No Hit
CCGTAGTAGTAGTAGTAATCATCCGGTCTCGCACAGTAATACACGGCCGT13350.1546768007971359No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT13080.15154850594955335No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA12940.14992642713969573No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT12530.14517605348225562No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG12450.14424915130519414No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12060.13973050319201935No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11950.1384560126985598No Hit
GTGGTAACACAGGCTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACC11500.13324218795258894No Hit
CCATGTAGGCTGTGCTTATGGAGGTGTTCCTGGTCATGGTGACTCTGCCC11340.13138838359846597No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAA11150.12918699092794494No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11070.12826008875088346No Hit
CTGTTAGGGTTCATCCATCCCATCCACTCAAGCCCTTGTCCAGTGGCCTG11070.12826008875088346No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA10590.12269867568851453No Hit
CCCTAACAGTGGTAACACAGGCTATGCACAGAAGTTCCAGGGCAGAGTCA10400.12049728301799349No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10290.11922279252453395No Hit
ATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTG10240.1186434786638705No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC10210.11829589034747245No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA10160.11771657648680903No Hit
CATGTAGGCTGTGCTTATGGAGGTGTTCCTGGTCATGGTGACTCTGCCCT10100.11702139985401291No Hit
GCTCTATGCTGCATCCAGATTGGAAAGTGGGGTCCCATCCAGGTTCAGTG9920.11493586995562456No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG9760.11308206560150158No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9750.11296620282936891No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA9640.11169171233590935No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9590.11111239847524591No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC9530.1104172218424498No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9500.11006963352605174No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG9440.10937445689325562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9400.10891100580472488No Hit
TCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGCT9370.10856341748832683No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9340.10821582917192878No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9330.10809996639979608No Hit
CTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT9130.10578271095714237No Hit
GGATACACCTTCACCAGTTATGATATCAACTGGGTGCGACAGGCCACTGG8760.10149578838823296No Hit
GTGTAATCCGTCCCAGATCCACTGCCACTGAACCTGGATGGGACCCCACT8720.10103233729970224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG9250.066.1705253
TCAACGC9400.065.114624
CAACGCA9650.063.4277045
AACGCAG10250.059.701026
GTATCAA10550.058.02691
ACGCAGA11100.055.1293227
CGCAGAG11150.054.8852848
GTCATAG14800.041.8504031
TCATAGG15950.038.8284722
ATAGGTG15350.038.4674034
ATGCAAC3800.037.906121
TAGGTGG16000.037.8047755
CATAGGT16250.036.7800373
GTACATG17500.036.628141
CTATACC1201.00182115E-536.004554
GAATACG2800.035.998299
GTGGTCA16700.035.7827348
CAGAGCG12800.034.8733449
AGGTGGT17350.034.855116
GTATTAT18250.033.9389571
Produced by FastQC (version 0.11.5)