FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900385
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAGTTCCAATGGCAAGAACTATTTAGCTTGGTTTCAACAGAA26770.2973172587282107No Hit
GTAATAGACAGCCGTGTCCTCGCCTCTCAGACTGTTCATGTCCAGATATA24660.2738828390077578No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23050.2560015993158482No Hit
GTCTATTACTGTCAGCAATATTTTAAAACTCGCCTCACTTTCGGCGGAGG21520.2390088684285056No Hit
GTCTATTACTGTACGACTCATCCCGGGACCAACAGCTGGGACAAAGATTA18750.20824425107037545No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18480.20524553385496205No Hit
CTATTACTGTCAGCAATATTTTAAAACTCGCCTCACTTTCGGCGGAGGGA16960.1883638665681903No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14150.15715499480777667No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13960.1550447863969302No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12450.1382741827107293No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12270.1362750379004537No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12270.1362750379004537No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12250.13605291069931194No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11890.13205462107876076No Hit
CTCCTAAGGTCCTCATTAAGTGGGCTTCCGCCCGGGAATCCGGGGTCCCT11660.12950015826563083No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11390.1265014410502174No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11360.1261682502485048No Hit
TTTAAGAGGTGTCCAGTGTCAGGCGCAGTTAGTGGAGTCTGGGGGAGGCG11040.12261421503023708No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10680.11861592540968585No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10270.11406231778628032No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9890.10984190096458737No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9880.1097308373640165No Hit
CTCCTGTGCAGCGTCTGGATTCACATTCATAAGTTATGGCTTCCACTGGG9580.10639892934689049No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9330.10362233933261882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.077.344711
GGTATCA5750.071.511521
ATCAACG18200.059.728453
TCAACGC18750.057.9764184
TATCAAC19100.056.955142
CAACGCA19150.056.3894965
AACGCAG20750.052.0413866
CGCAGAG23800.045.372228
ACGCAGA27550.039.1963277
GCAGAGT28750.037.5603079
TAGACAG15850.035.4275445
CAGCCGT13900.033.1467449
TATATAG15650.032.6602675
ATAGACA16350.032.1425864
GTACATG23100.030.6043451
ACATGGG22900.030.4938283
TAGTTCC16300.030.4745439
TACATGG23250.030.366342
ATATAGT17850.028.6349126
TATAGTT17900.028.1527447
Produced by FastQC (version 0.11.5)