FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884311
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGGTCCTCCGCTGACGTTCGGCCAAGGGACCAAGGTGGAAA29250.3307659861745472No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGTT20650.2335151321198085No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19080.21576119713539693No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14800.1673619348848991No Hit
GTAATAGACGGCCGTGTCCTCGGCTCCCAGGCTCTTCATTTGTAGAAACA11650.13174098252764016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.12393829772557392No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT10230.11568328336976472No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9630.10889834006362015No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9480.10720210423708401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16000.065.435711
GGTATCA8950.065.347471
TCAACGC19800.052.7218674
ATCAACG20150.051.8061033
CAACGCA20900.049.9470375
TATCAAC22200.047.3840072
AACGCAG22800.045.7847826
ACGCAGA24950.041.83947
CGCAGAG26250.040.041618
GCAGAGT29550.034.8390669
TATACCG900.003754935231.996725
TATAGTG16500.031.8512845
ATTATAG17850.030.2489993
TAGACGG6450.029.020285
ATAGTGG17650.028.9602056
ATATTGC14950.028.4118026
ATAGACG6750.027.7304924
TATAATA18300.027.5599782
CAGAGTA31200.026.7664879
TTATAGT19150.026.6917824
Produced by FastQC (version 0.11.5)