FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665905
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA46310.6954445453931116No Hit
TTGTATTACTGTGCAAAAGCTAGCAGCAGCTGGTACCTTGACTACTGGGG30010.4506648846306906No Hit
GTATGTGCTACCACCATCCCCACTAATAAGAGAGACCCACTCCAGACCCT27020.40576358489574343No Hit
CTCTTATTAGTGGGGATGGTGGTAGCACATACTATGCAGACTCTGTGAAG24600.36942206470892996No Hit
AGCACATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA23950.3596609125926371No Hit
GTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCT20840.3129575540054512No Hit
CTAATAAGAGAGACCCACTCCAGACCCTTCCCTGGAGCTTGACGGACCCA17140.25739407272809184No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTACTGTGCAAAAGCTAGCAGC17070.2563428717309526No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16850.253039097168515No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16370.2458308617595603No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16220.24357828819426192No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15820.23757142535346634No Hit
CAGTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA15570.23381713607796908No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15410.23141439094165084No Hit
GTATTACTGTGCAAAAGCTAGCAGCAGCTGGTACCTTGACTACTGGGGCC15050.2260082143849348No Hit
ATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACA14870.22330512610657677No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCG14740.2213528956833182No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13820.2075371111494883No Hit
ATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACAGGG13590.20408316501603083No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCA13590.20408316501603083No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC13370.20077939045359322No Hit
GCATAGTATGTGCTACCACCATCCCCACTAATAAGAGAGACCCACTCCAG13230.19867698845931475No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13190.1980763021752352No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTG13160.19762578746217552No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13130.19717527274911587No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12950.19447218447075784No Hit
CTCCAGACCCTTCCCTGGAGCTTGACGGACCCAGTGCATGGCATAATCAT12790.1920694393344396No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGCCATGCACTGGG12530.18816497848792246No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12500.18771446377486278No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12440.18681343434874345No Hit
GGTCTGGAGTGGGTCTCTCTTATTAGTGGGGATGGTGGTAGCACATACTA12370.1857622333516042No Hit
GTGGTAGCACATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC12150.18245845878916664No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA12050.18095674307896772No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGCC12000.18020588522386827No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11630.17464953709613235No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11470.17224679195981407No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11030.16563924283493892No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT10970.16473821340881958No Hit
GTCTGCATAGTATGTGCTACCACCATCCCCACTAATAAGAGAGACCCACT10880.16338666926964054No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA10820.1624856398435212No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10790.16203512513046156No Hit
ATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAA10700.16068358099128255No Hit
GTGCTACCACCATCCCCACTAATAAGAGAGACCCACTCCAGACCCTTCCC10510.15783032114190462No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10340.1552774044345665No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10070.15122277201702947No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9960.14957088473581065No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9870.14821934059663164No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9820.1474684827415322No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCA9590.14401453660807473No Hit
GATCAGCACTGAACACAGAGAACTCACCATGGAGTTTGGACTGAGCTGGG9440.14176196304277638No Hit
GCCATGCACTGGGTCCGTCAAGCTCCAGGGAAGGGTCTGGAGTGGGTCTC9440.14176196304277638No Hit
GTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAC9380.14086093361665705No Hit
GTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTACAGCCTGGGG9340.14026024733257747No Hit
GAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGC9140.13725681591217967No Hit
GCACTGAACACAGAGAACTCACCATGGAGTTTGGACTGAGCTGGGTTTTC9110.13680630119912No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA9010.13530458548892108No Hit
GTCTGGGGGAGGCGTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTG8770.13170046778444375No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8760.13155029621342385No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8680.1303489236452647No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG8560.12854686479302604No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8470.12719532065384703No Hit
GTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGC8300.12464240394650888No Hit
GAACACAGAGAACTCACCATGGAGTTTGGACTGAGCTGGGTTTTCCTTGT8240.12374137452038955No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG8170.12269017352325032No Hit
CTGTAGCAGCCACAGAGGTCACGGTCAGGTGGTCCTTGAACAGCGCTCTT7920.11893588424775306No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7770.1166833106824547No Hit
TCCCTGGCCCCAGTAGTCAAGGTACCAGCTGCTGCTAGCTTTTGCACAGT7650.11488125183021602No Hit
GCCTTGTATTACTGTGCAAAAGCTAGCAGCAGCTGGTACCTTGACTACTG7580.11383005083307679No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG7580.11383005083307679No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7470.11217816355185799No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGC7190.10797335956330106No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7100.10662181542412205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.10647164385310216No Hit
GTATCAACGCAGAGTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGA7090.10647164385310216No Hit
CGCTGTAGCAGCCACAGAGGTCACGGTCAGGTGGTCCTTGAACAGCGCTC7080.10632147228208227No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7080.10632147228208227No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC7080.10632147228208227No Hit
GAGTTACAGTACCCGTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCA7030.10557061442698283No Hit
GTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG7000.10512009971392317No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6980.10481975657188339No Hit
TGATTATGCCATGCACTGGGTCCGTCAAGCTCCAGGGAAGGGTCTGGAGT6920.10391872714576404No Hit
GCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCACAGAGG6760.1015159820094458No Hit
GTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGA6700.10061495258332645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT400.00621891453.9978079
CCGTTCA450.00990643347.998059
TCAACGC19200.035.623554
ATCAACG19850.034.8223573
CTTAGAC1051.9295666E-434.2868923
CAACGCA20200.033.860015
TATCAAC20300.033.695742
AACGCAG20500.033.36456
GTATCAA21650.032.9273951
CGCAGAG21400.031.9613198
ACGCAGA21500.031.812667
GTATAAC1153.289776E-431.3077771
GTGCTAG2559.640644E-1131.0622251
GATATAC950.004888053530.3168333
GTAATAC23550.030.270831
GCAGAGT23450.029.7813039
TCAGGGG6050.029.7530923
AGGCGGT24000.029.3988049
CAAGGCG24100.029.276827
TAATACA24550.029.0356242
Produced by FastQC (version 0.11.5)