FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665905
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA47450.7125641044893791No Hit
TTGTATTACTGTGCAAAAGCTAGCAGCAGCTGGTACCTTGACTACTGGGG29910.44916316892049163No Hit
GTATGTGCTACCACCATCCCCACTAATAAGAGAGACCCACTCCAGACCCT25440.3820364766746007No Hit
CTCTTATTAGTGGGGATGGTGGTAGCACATACTATGCAGACTCTGTGAAG25340.3805347609644018No Hit
AGCACATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA24150.3626643440130349No Hit
GTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCT21370.3209166472695054No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTACTGTGCAAAAGCTAGCAGC17300.25979681786441006No Hit
CTAATAAGAGAGACCCACTCCAGACCCTTCCCTGGAGCTTGACGGACCCA16620.24958515103505755No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16100.24177622934202325No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15600.23426765079102874No Hit
CAGTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA15390.23111404779961106No Hit
GTATTACTGTGCAAAAGCTAGCAGCAGCTGGTACCTTGACTACTGGGGCC15270.22931198894737237No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14580.21895015054699996No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCG13980.20993985628580655No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCA13930.20918899843070707No Hit
ATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACA13750.20648591015234907No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC13730.2061855670103093No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13630.20468385130011038No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13460.20213093459277223No Hit
ATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACAGGG13410.20138007673767278No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTG13280.19942784631441424No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA12940.19432201289973794No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12860.19312064033157883No Hit
CTCCAGACCCTTCCCTGGAGCTTGACGGACCCAGTGCATGGCATAATCAT12800.1922196109054595No Hit
GGTCTGGAGTGGGTCTCTCTTATTAGTGGGGATGGTGGTAGCACATACTA12570.18876566477200202No Hit
GCATAGTATGTGCTACCACCATCCCCACTAATAAGAGAGACCCACTCCAG12430.18666326277772355No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12290.18456086078344508No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12260.18411034607038543No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12230.18365983135732575No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGCCATGCACTGGG12100.18170760093406715No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGCC11930.17915468422672903No Hit
GTGGTAGCACATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC11880.1784038263716296No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11740.1763014243773511No Hit
GTCTGCATAGTATGTGCTACCACCATCCCCACTAATAAGAGAGACCCACT11350.1704447331075754No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT11210.16834233111329694No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11070.16623992911901847No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA10830.16263581141454111No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10730.1611340957043422No Hit
GTGCTACCACCATCCCCACTAATAAGAGAGACCCACTCCAGACCCTTCCC10670.16023306627822287No Hit
ATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAA10580.15888152213904386No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10570.15873135056802395No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG10460.15707946328680517No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10440.1567791201447654No Hit
GATCAGCACTGAACACAGAGAACTCACCATGGAGTTTGGACTGAGCTGGG10400.15617843386068583No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG10390.15602826228966596No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10080.15137294358804934No Hit
GCCATGCACTGGGTCCGTCAAGCTCCAGGGAAGGGTCTGGAGTGGGTCTC9860.14806916902561176No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCA9800.14716813959949243No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9750.14641728174439297No Hit
GTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAC9400.14116127675869683No Hit
GAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGC9150.13740698748319957No Hit
GCACTGAACACAGAGAACTCACCATGGAGTTTGGACTGAGCTGGGTTTTC9150.13740698748319957No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG9070.13620561491504043No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA8880.1333523550656625No Hit
GTCTGGGGGAGGCGTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTG8860.13305201192362273No Hit
GTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTACAGCCTGGGG8640.12974823736118515No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG8640.12974823736118515No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8600.1291475510771056No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8470.12719532065384703No Hit
GAACACAGAGAACTCACCATGGAGTTTGGACTGAGCTGGGTTTTCCTTGT8300.12464240394650888No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8140.12223965881019065No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG8050.12088811467101163No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7790.11698365382449448No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC7710.11578228125633536No Hit
CCCTCATGCTGCCACCCCCGACTGTCACTGCACCGACCGGCCCTCGAGGA7700.11563210968531547No Hit
GCCTTGTATTACTGTGCAAAAGCTAGCAGCAGCTGGTACCTTGACTACTG7660.11503142340123591No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7640.11473108025919614No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7630.11458090868817625No Hit
TCCCTGGCCCCAGTAGTCAAGGTACCAGCTGCTGCTAGCTTTTGCACAGT7550.11337953612001712No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7550.11337953612001712No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7450.11187782040981821No Hit
GTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGC7450.11187782040981821No Hit
TGATTATGCCATGCACTGGGTCCGTCAAGCTCCAGGGAAGGGTCTGGAGT7420.11142730569675854No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG7280.10932490370248009No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT7140.10722250170820162No Hit
GTATCAACGCAGAGTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGA7130.10707233013718173No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7060.1060211291400425No Hit
GGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGC6860.10301769771964468No Hit
GTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCG6840.1027173545776049No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6810.10226683986454524No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6770.10166615358046568No Hit
GTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGC6740.101215638867406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)