FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639318
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGCCACAGACGTCAGGCTCAGGGAGA44050.6890154821231375No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33800.5286883835587297No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26230.4102809556433574No Hit
GTCTATTACTGTGCGAGACATGGGTGGCCCCATGTCTCCAGTGACTATAA25980.40637053860520117No Hit
TCCTTATAGTCACTGGAGACATGGGGCCACCCATGTCTCGCACAGTAATA23690.3705511185356896No Hit
GTCTCTGGTGTCTCCCTCAGCACTACTACTGACTCCTGGGCCTGGATCCG22480.35162470007101315No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG20870.3264416143452867No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20110.3145539465492916No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17940.2806115266580951No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT17770.2779524430721488No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC16240.2540206907986323No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14870.23259160542953586No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCACTTTGTTCATG14550.22758627162069578No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13370.20912910320059816No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT12650.19786710213070802No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12540.19614651863391927No Hit
CTATAAGGATGTTCTTGATATGTGGGGCCAAGGGGCAATGGTCACCGTCT11920.18644868437929168No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC11600.18144335057045163No Hit
CACCTGCACTGTCTCTGGTGTCTCCCTCAGCACTACTACTGACTCCTGGG10890.1703377661820878No Hit
ATAATAATCAGCCTCATCGTCAGGCTGGAGCCCAGAGACGGTCAGGGAGG10520.1645503489656165No Hit
CCCTGAAGAGACGGTGACCATTGCCCCTTGGCCCCACATATCAAGAACAT10440.16329901551340648No Hit
ACTACAACCCGTCCCTCAGGAGTCGAGTCACCATATCCGTGGACACGTCC10420.16298618215035396No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9930.15532176475556767No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT9900.1548525147109889No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9900.1548525147109889No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC9730.15219343112504263No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA9530.14906509749451757No Hit
ATCTATTCTTCTGGGAGCCCCTACTACAACCCGTCCCTCAGGAGTCGAGT9490.1484394307684126No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC9450.1478137640423076No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC9450.1478137640423076No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCAC9410.14718809731620258No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9160.1432776802780463No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA8900.13921084655836377No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8890.13905442987683753No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT8680.13576967956478622No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8650.13530042952020746No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG8400.13139001248205118No Hit
GGTTATAACTATGTCACCTGGTACCAACAACACCCAGGCAAAGCCCCCAA8200.12826167885152617No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG8170.1277924288069474No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGCCACAGACGTCAGGCTCAGGGA7910.12372559508726487No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7910.12372559508726487No Hit
TCTCTGGGCTCCAGCCTGACGATGAGGCTGATTATTATTGCTGCTCATAT7860.12294351167963362No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTATGTCACCT7810.12216142827200235No Hit
ATCCATATGAGCAGCAATAATAATCAGCCTCATCGTCAGGCTGGAGCCCA7800.1220050115904761No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT7800.1220050115904761No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC7710.12059726145673982No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7660.11981517804910857No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7630.11934592800452984No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7500.11731251114468855No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAGCACTTTGTTCATGT7410.11590476101095229No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT7400.11574834432942605No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA7360.11512267760332104No Hit
GTAGTAGGGGCTCCCAGAAGAATAGATAGTCCCAATCCACTCTAGTCCCT7350.1149662609217948No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7250.11340209410653228No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCA7200.11262001069890103No Hit
GATTATTATTGCTGCTCATATGGATACATGAACAAAGTGCTGTTCGGCGG7140.1116815106097435No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7090.11089942720211225No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG7070.11058659383905976No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6950.10870959366074473No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC6900.10792751025311348No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC6890.10777109357158722No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG6800.10636334343785095No Hit
GTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGC6780.10605051007479846No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG6680.10448634325953596No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT6670.10432992657800969No Hit
GTTATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG6600.10323500980732594No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6530.10214009303664218No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6440.1007323429029059No Hit
ACTACTGACTCCTGGGCCTGGATCCGCCAGCCCCCCGGGAAGGGACTAGA6400.1001066761768009No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCG400.00622051453.9940459
GTGCTAG2950.053.722621
GAATACG2950.053.688999
ATCAACG10850.045.8008843
CTAGAGA3950.045.5824284
TCAACGC10850.045.1371044
TAGGTGG12800.045.012645
GCTAGAG3700.044.7693333
ATAGGTG12500.044.364464
GTCATAG12550.044.198091
CAGAGCG10950.044.049949
GCACACA10700.043.7540322
TATCAAC11600.043.4638862
GTGGTCA12950.043.362018
GTATCAA12100.042.8650781
CAACGCA11650.042.0375635
AACGCAG11850.041.3118976
CCCCGTT4300.040.1816187
AGAATAC4000.039.5956348
ACAGAGC12400.039.479528
Produced by FastQC (version 0.11.5)