FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016356
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19480.191665125212032No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTCACCACTGGGGCTGGATCCG18660.18359708606039618No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15130.14886516141981745No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14930.14689734699258922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.14640539338578215No Hit
ATAATAAACGGCCGTGTCCGCGGCGGTCATAGAATTCAGCTTCAGGAAGA14540.1430601088594941No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC13990.1376486191846164No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12560.12357874602993439No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11930.1173801305841654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27050.056.5828971
ATCAACG28200.053.3573463
TCAACGC28700.052.4277764
CAACGCA29300.051.8455925
TATCAAC30300.049.9485172
AACGCAG30850.049.2407076
GGTATCA11750.048.5406721
TAGCACG605.1188434E-447.9960754
ACGCAGA33900.044.81057
CGCAGAG34000.044.6787078
GCAGAGT38750.038.830389
GAATACG4850.034.141549
GTGCTAG6450.033.57981
TGCACCG3200.031.4974275
GTGTAGA5850.030.8532351
AGCGTAC1255.369482E-428.7976475
GCACCGA4500.027.1977776
GTATAGA3204.3655746E-1127.0737131
GTACATG26850.026.0821881
ACCGACC3900.025.8440428
Produced by FastQC (version 0.11.5)