FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535519
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA22410.4184725471925366No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG17220.3215572183246533No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10910.20372759883402833Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9080.16955514183437Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8220.15349595439190766No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7350.13725003221174226Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAG6690.1249255395233409No Hit
TGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCACTG6510.121564314244686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5590.10438471837600533No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5570.10401124890059923No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)