FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748824
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCAGCTTTCAGGCTGTTCATTTGCAGATACA29220.3902118521842249No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21200.28311058406247663No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20800.27776887492922236No Hit
GTGTATTACTGTGCGAGAGAGGGTTACGATTCTTGGAGTGGATCTCATGC16290.21754110445178038No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15070.20124889159535483No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG13760.18375479418394708No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13450.17961496960567502No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12800.17093469226413682No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG12520.16719549587085886No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10820.14449323205452816No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT9880.13194021559138064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.12966998920974754No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9070.1211232545965407No Hit
ATACACAGCCGTGTCGTCAGCTTTCAGGCTGTTCATTTGCAGATACAGCG8680.11591508819161779No Hit
CCCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAGCAT8570.11444611817997287No Hit
CAGTAATACACAGCCGTGTCGTCAGCTTTCAGGCTGTTCATTTGCAGATA7910.1056322981101033No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC7560.10095830261850582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16400.063.779091
ATCAACG16800.059.993213
TCAACGC16850.059.815194
CAACGCA17100.058.94075
AACGCAG17300.058.2593086
GGTATCA6500.057.7090841
TATCAAC18250.056.0604972
ACGCAGA19800.050.9033287
CGCAGAG20750.048.5728158
GCAGAGT24950.040.6847769
GTCATAG10300.037.819061
CCCCGTT3250.037.6572767
ATAGGTG10150.034.045414
CCCGTTG3400.033.878528
TCATAGG11350.033.64432
TAGGTGG11050.033.227015
CTAGAGA4450.032.355894
CAGAGCG9500.031.8279769
GAATACG2757.2759576E-1231.4146279
GTGTAGA4700.030.6963211
Produced by FastQC (version 0.11.5)