FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748824
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCAGCTTTCAGGCTGTTCATTTGCAGATACA23530.3142260397636828No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20470.27336196489428755No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16360.21847590355009988No Hit
GTGTATTACTGTGCGAGAGAGGGTTACGATTCTTGGAGTGGATCTCATGC13930.18602502056558015No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG13540.18081685416065724No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11340.15143745392775873No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11160.1490336848177943No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10390.13875089473627983No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG9830.13127250194972384No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9490.1267320491864577No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9190.122725767336517No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7890.10536521265344059No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG400.006224835353.9854779
TCAACGC10850.052.4098634
ATCAACG11300.050.3227463
CAACGCA11450.049.0348435
AACGCAG11850.047.987096
GTATCAA12450.047.4755061
ACGCAGA12500.044.3400737
TATCAAC13400.043.510682
CGCAGAG13150.042.1483578
TAGCGCG907.8044075E-539.9892464
ATAGGTG11000.039.2621654
TCATAGG11500.037.5551152
TAGGTGG11750.037.368675
GTCATAG11650.037.1236271
GGTATCA6150.035.1618081
GCAGAGT15600.035.0674869
CATAGGT13400.032.7673033
CCGTTGA3450.031.2959279
ATTACGG950.00489569830.3076368
GTGCTAG4100.029.8875371
Produced by FastQC (version 0.11.5)