FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705568
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG15710.22265749013560707No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA10630.15065876003446868No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10480.14853281327951381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.13591886253344823No Hit
GTGTATTACTGTGCGAGAGAGGATCCTGACTCTCACTACTACTACGGTAT9590.13591886253344823No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA9060.12840718399927434No Hit
TCCCTATCCTTGGTATAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTC9050.1282654542156107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8390.11891128849380925No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7710.10927366320468049No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7430.10530522926209805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14200.061.3807531
TCAACGC15700.053.6582684
ATCAACG16250.051.849493
CAACGCA16350.051.084685
TATCAAC17700.048.4156462
AACGCAG17650.047.322076
ACGCAGA20250.041.2432257
CGCAGAG20400.040.939978
GTATAAG3800.026.5386961
ATACGAG2207.9337406E-726.1866133
CAGAGTA24250.026.1270649
GTATTAG3109.240466E-1025.5603121
GCAGAGT32750.025.5015359
TACTAGG1701.0791606E-425.4164182
GTACATG19200.024.0112021
GGTATCA14000.023.6681861
TACGAGT2452.0285424E-623.5111754
ACGAGTG2452.0285424E-623.5111755
GTGTAGA3453.1905074E-922.9672371
TTAGGCT1902.2968056E-422.7377824
Produced by FastQC (version 0.11.5)